Analyzing Microbes Manual of Molecular Biology Techniques /

Podrobná bibliografie
Korporativní autor: SpringerLink (Online service)
Další autoři: Arora, Dilip Kumar (Editor), Das, Surajit (Editor), Sukumar, Mesapogu (Editor)
Shrnutí:XI, 352 p. 80 illus., 62 illus. in color.
text
Jazyk:angličtina
Vydáno: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 2013.
Vydání:1st ed. 2013.
Edice:Springer Protocols Handbooks,
Témata:
On-line přístup:https://doi.org/10.1007/978-3-642-34410-7
Médium: Elektronický zdroj Kniha
Obsah:
  • Microbial DNA extraction, purification and quantitation
  • Fluorescent based detection, quantitation and expression of wviral gene by qRT-PCR
  • Restriction enzymes and their role in microbiology
  • Genetic fingerprinting techniques  for molecular characterization of microbes
  • Agarose gel electrophoresis and Polyacrylamide agarose gel electrophoresis: Methods and principles
  • Molecular identification of microbes
  • Preservation and maintenance of microbial cultures
  • Microbes from extreme environment: Molecular identification procedures
  • ELISA based identification and detection of microbes
  • Analysis of microbial diversity and construction of metagenomic library
  • Bioinformatics tools for interpretation of data used in molecular identification
  • Molecular phylogenetics of microbes
  • Microarray technology: Basic concept, protocols and applications
  • Microarray analysis of different functional genes of microorganisms
  • DNA cloning and sequencing
  • Biological sequence analysis: Algorithms and statistical methods.