Mitochondrial Bioenergetics Methods and Protocols /

Библиографические подробности
Автор-организация: SpringerLink (Online service)
Другие авторы: Palmeira, Carlos M. (Редактор), Moreno, António J. (Редактор)
Примечания:XI, 333 p. 83 illus., 25 illus. in color.
text
Язык:английский
Опубликовано: Totowa, NJ : Humana Press : Imprint: Humana, 2012.
Издание:1st ed. 2012.
Серии:Methods in Molecular Biology, 810
Предметы:
Online-ссылка:https://doi.org/10.1007/978-1-61779-382-0
Формат: Электронный ресурс Книга
Оглавление:
  • Overview of Mitochondrial Bioenergetics.- Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells.-  High-Resolution Respirometry.  OXPHOS Protocols for Human Cells and Permeabilized Fibres from Small Biopsies of Human Muscle
  • High Throughput Analysis of Mitochondrial Oxygen Consumption. – Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices. - Mitochondrial Membrane Potential (DY) Fluctuations Associated with the Metabolic States of Mitochondria. - Safranine as a Fluorescent Probe for the Evaluation of Mitochondrial Membrane Potential in Isolated Organelles and Permeabilized Cells
  • Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells.-Phenomenological Kinetic and Control Analysis of Oxidative Phosphorylation in Isolated Mitochondria.-Expression of Uncoupling Proteins in a Mammalian Cell Culture System (HEK293) and Assessment of their Protein Function.-Measurement of Proton Leak and Electron Leak in Isolated Mitochondria.-Relation between Mitochondrial Membrane Potential and ROS Formation
  • Use of a Calcium-Sensitive Electrode for Studies on Mitochondrial Calcium Transport .-Imaging Mitochondrial Calcium Signalling with Fluorescent Probes and Single or Two Photon Confocal Microscopy
  • Mitochondrial Permeability Transition Pore and Calcium Handling.- Imaging of Mitochondrial pH Using SNARF-1
  • Redox Equivalents and Mitochondrial Bioenergetics.-NMR Methodologies for Studying Mitochondrial Bioenergetics.-Computational Modeling of Mitochondrial Function.