Transgenic Mouse Methods and Protocols

গ্রন্থ-পঞ্জীর বিবরন
সংস্থা লেখক: SpringerLink (Online service)
অন্যান্য লেখক: Hofker, Marten H. (Editor), van Deursen, Jan (Editor)
সংক্ষিপ্ত:XI, 352 p.
text
ভাষা:ইংরেজি
প্রকাশিত: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
সংস্করন:2nd ed. 2011.
মালা:Methods in Molecular Biology, 693
বিষয়গুলি:
অনলাইন ব্যবহার করুন:https://doi.org/10.1007/978-1-60761-974-1
বিন্যাস: বৈদ্যুতিক গ্রন্থ
সূচিপত্রের সারণি:
  • Introduction: Strategies for developing genetically modified mice
  • Genetic Modification of the Mouse; General Technology: Pronuclear and Blastocyst Injection
  • Generation of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos
  • Cryopreservation of mouse spermatozoa and In vitro fertilization
  • Autopsy and histological analysis of the transgenic mouse
  • Transgene design
  • Inducible Transgenic Mouse Models
  • Lentiviral transgenesis
  • Transgenesis in Mouse Embryonic Stem Cells
  • Engineering BAC Reporter Gene Constructs for Mouse Transgenesis
  • Targeting Vector Construction Through Recombineering
  • Generating Conditional Knockout Mice
  • Hypomorphic mice
  • MICER Targeting Vectors for Manipulating the Mouse Genome
  • Knockin approaches
  • Generation of a series of knockin alleles using RMCE in ES cells
  • Selection of targeted mutants from a library of randomly mutagenized ES cells
  • Generation of genetically modified rodents using random ENU mutagenesis
  • Bone marrow transplantations to study gene function in hematopoietic cells
  • Adenovirus Mediated Gene Transfer.