The Protein Protocols Handbook

Bibliographic Details
Corporate Author:	SpringerLink (Online service)
Other Authors:	Walker, John M. (Editor)
Summary:	LXX, 1984 p. text
Language:	English
Published:	Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edition:	3rd ed. 2009.
Series:	Springer Protocols Handbooks,
Subjects:	Proteins . Post-translational modification. Protein Biochemistry. Post-translational Modifications.
Online Access:	https://doi.org/10.1007/978-1-59745-198-7
Format:	Electronic Book

Table of Contents:

Protein Determination by UV Absorption
The Lowry Method for Protein Quantitation
The Bicinchoninic Acid (BCA) Assay for Protein Quantitation
The Bradford Method for Protein Quantitation
Ultrafast Protein Determinations Using Microwave Enhancement
The Nitric Acid Methods for Protein Estimation in Biological Samples
Quantitation of Tryptophan in Proteins
Kinetic Silver Staining of Proteins
Quantitation of cellular proteins by flow cytometry
Quantitation of cellular proteins by laser scanning cytometry
Protein Solubility in 2D Electrophoresis: Basic principles and issues
Mouse and Human Tissues Sample Preparation for 2-D Electrophoresis
Plant Protein sample preparation for 2DE
Preparation of bacterial samples for 2-D PAGE
Preparation of bodily fluids for 2-D PAGE
Immunoaffinity Depletion of high abundance plasma and serum proteins
Preparation of Yeast samples for 2D PAGE
Membrane Protein Preparation Using Aqueous Polymer Two Phase Systems
Subcellular fractionation of small sample amounts
Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins
SDS Polyacrylamide Gel Electrophoresis of Proteins
Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins
SDS-Polyacrylamide Gel Electrophoresis of Peptides
Blue native gel electrophoresis (BN-PAGE)
Separation of proteins by gel electrophoresis in the Tris-Taurine-HCl system
Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins: M -Based Separation of Proteins with Retained Native Activity
Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Basic Proteins
Acid-Urea-Triton Polyacrylamide Gel Electrophoresis of Histones
Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels
Serial immobilized pH gradient isoelectric focusing over pH 4-9
Radiolabelling of Eukaryotic Cells and Subsequent Preparation for 2-D
Two-Dimensional PAGE Using Carrier Ampholyte pH Gradients in the First Dimension
Vertical agarose electrophoresis and electroblotting of high molecular weight proteins
2D PAGE of high molecular weight proteins
Casting immobilised pH gradients
Nonequilibrium pH Gel Electrophoresis
Microchip capillary electrophoresis
Protein Separations in Microfluidic Chips
Difference gel electrophoresis (DIGE)
Comparing 2-D Electrophoresis Gels Across Internet Databases
Quantification of Radiolabeled Proteins in Polyacrylamide Gels 25.4.07
Differential ProteoTope radioactive quantification of protein abundance ratios
Quantitation of Proteins on Polyacrylamide Gels
Using SDS-PAGE and scanning laser densitometry to measure proteins
Rapid and Sensitive Staining of Unfixed Proteins in Polyacrylamide Gels with Nile Red
Zinc reverse staining technique
Protein Staining with Calconcarboxylic Acid in Polyacrylamide Gels
Detection of Proteins in Polyacrylamide Gels by Silver Staining
Background-free Protein Detection on Polyacrylamide Gels and on Electroblots Using Transition Metal Chelate Stains
Detection of Proteins in Polyacrylamide Gels by Fluorescent Staining
Detection of Glycoproteins in Gels and Blots
Staining of Glycoproteins/Proteoglycans on SDS-Gels
Detection of Proteins and Sialoglycoproteins in Polyacrylamide Gels Using Eosin X stain
Pro-Q Diamond phosphoprotein staining
Electroelution of Proteins from Polyacrylamide Gels
Autoradiography and Fluorography of Acrylamide Gels
Proteolytic Activity Detection by Two-Dimensional Zymography
Protein Blotting by Electroblotting
Protein Blotting by the Semi-dry Method
Protein Blotting by the Capillary Method
Western Blotting of basic proteins electrophoretically resolved on acid-urea-Triton-polyacrylamide gels
Immunoblotting of 2-DE Separated Proteins
High efficiency blotting of high-molecular weight proteins
Alkaline Phosphatase Labeling of IgG Antibody
b-Galactosidase Labeling of IgG Antibody
Horseradish Peroxidase Labeling of IgG Antibody
Digoxigenin (DIG) Labelling of IgG Antibody
Conjugation of fluorochromes to antibodies
Coupling of Antibodies with Biotin
Preparation of Avidin Conjugates
MDPF Staining of Proteins on Western Blots
Copper Iodided Staining of Proteins and its silver enhancement
Detection of Proteins on Blots using Direct Blue 71
Detection of proteins on Western blots using colorimetric and radiometric vusialization of Secondary Ligands
Identification of Glycoproteins on Nitrocellulose Membranes Using Lectin Blotting
A Sensitive Method to Quantitatively Detect Total Protein on Membranes after Electrophoretic Transfer Using Avidin- or Streptavidin-Biotin
Detection and Quantification of Proteins on Immunoblots using Enhanced Chemiluminescence
Reutilization of Western Blots After Chemiluminescent Detection or Autoradiography
The use of quantum dot luminescent probes for Western blot analysis
The use of infrared fluorescent dyes in quantitative immunoblotting
The use of infrared fluorescent dyes in immunofluorescence microscopy
Carboxymethylation of Cysteine Using Iodoacetamide/Iodoacetic Acid
Performic Acid Oxidation
Succinylation of Protein
Pyridylethylation of Cysteine Residues
Side-Chain Selective Chemical Modifications of Proteins
Nitration of Tyrosines
Ethoxyformylation of Histidine
Modification of Arginine Side Chains with p-Hydroxyphenylglyoxal
Amidination of Carboxyl Groups
Amidination of Lysine Side Chains
Modification of tryptophan with 2-Hydroxy-5-Nitrogenzylbromide
Modification of Sulhydryl Groups with DTNB
Chemical Cleavage of Proteins at Methionyl-X Peptide Bonds
Chemical Cleavage of Proteins at Tryptophanyl-X Peptide Bonds
Chemical Cleavage of Proteins at Aspartyl-X Peptide Bonds
Chemical Cleavage of Proteins at Cysteinyl-X Peptide Bonds
Chemical Cleavage of Proteins at Asparaginyl-Glycyl Peptide Bonds
Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels
On-PVDF protein digestions for N-terminal sequencing and peptide mass fingerprinting
Enzymatic Digestion of Proteins on PVDF membranes
Reverse Phase HPLC Separation of Enzymatic Digests of Proteins
Peptide Mapping by Two-Dimensional Thin-Layer Electrophoresis-Thin-Layer Chromatography
Peptide Mapping by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis
Peptide Mapping by High-Performance Liquid Chromatography
Production of Protein Hydrolysates Using Enzymes
Amino Acid Analysis by Precolumn Derivatization with 1- Fluoro-2,4-Dinitrophenyl-5-L-Alanine Amide (Marfey’s Reagent)
Amino acid analysis in protein hydrolysates using anion exchange chromatography and IPAD detection
Validation of amino acid analysis
Molecular Weight Estimation for Native Proteins Using High-Performance Size Exclusion Chromatography
Detection of Disulfide-Linked Peptides by HPLC
Detection of Disulfide-Linked Peptides by Mass Spectrometry
Diagonal Electrophoresis for Detecting Disulfide Bridges
Estimation of Disulfide Bonds Using Ellman’s Reagent
Quantitation of Cysteine Residues and Disulfide Bonds by Electrophoresis
N-Terminal sequencing of the N-terminally Modified Proteins
De-blocking of Proteins containing N-Terminal Pyroglutamic Acid
Detection and characterization of protein mutations by mass spectrometry
Peptide Sequencing by Nanoelectrospray Tandem Mass Spectrometry
Protein identification by Peptide mass fingerprinting using MALDI-TOFF Mass spectrometry
Protein ladder sequencing
Sequence Analysis with WinGene/WinPep
HPLC and MS of integral membrane proteins
Enrichment of Serum Peptides and Analysis by MALDI-TOF Mass Spectrometry
Computational Methods for the Analysis of MALDI-TOF Spectra to Discover Peptide Serum Biomarkers
Simple tools for complex N-glycan analysis
A Lectin-Binding Assay for the Rapid Characterization of the Glycosylation of Purified Gycoproteins
Chemical Methods of Analysis of Glycoproteins
Monosaccharide Analysis by HPAEC
Monosaccharide Analysis by GC
Determination ofmonosaccharide linkage and Substitution Patterns by GC.MS Methylation Analysis
Sialic Acid Analysis by HPAEC-PAD
Chemical Release of O-Linked Oligosaccharide Chains
O-Linked Oligosaccharide Profiling by HPLC
O-Linked Oligosaccharide Profiling by HPAEC-PAD
Release of N-Linked Oligosaccharide Chains by Hydrazinolysis
Enzymatic Release of O- and N-Linked Oligosaccharide Chains
N-Linked Oligosaccharide Profiling by HPLC on Porous Graphitized Carbon (PGC)
N-linked Oligosaccharide Profiling by HPAEC-PAD
HPAE-PAD analysis of monosaccharides released by exoglycosidase digestion
Microassay Analyses of Protein Glycosylation
Polyacrylamide gel electrophoresis of fluorophore-labeled carbohydrates from glycoproteins
HPLC Analysis of fluorescently labelled glycans
Glycoprofiling purified glycoproteins using Surface Plasmon Resonance
Sequencing Heparan Sulphate Saccharides
Analysis of glycoprotein heterogeneity by Capillary Electrophoresis and Mass Spectrometry
Affinity chromatography of oligosaccharides and glycopeptides with immobilized lectins
In-Gel Enzymatic Release of N-glycans
Analysis of N-Linked glycans by mass spectrometry
MS Analysis of Protein glycosylation
Mapping protein N-glycosylation by COFRADIC
Mass spectrometric analysis of O-linked glycans released directly from Glycoproteins in gels using ß-elimination
Glycopeptide analysis using LC/MS and LC/MSn
Identification of Vitamin K-Dependent Proteins Using a Gla-specific Monoclonal Antibody
The identification of protein S-Nitrocysteine
Detection of nitrotyrosine-containing proteins
Mass spectrometric determination of protein Ubiquitination
Detection of Sumoylated Proteins
Efficient enrichment of intact phosphorylated proteins by modified immobilized metal-affinity chromatography
Analyzing Protein Phospho.

The Protein Protocols Handbook

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