Signal Transduction Protocols

書誌詳細
団体著者: SpringerLink (Online service)
その他の著者: Dickson, Robert C. (編集者), Mendenhall, Michael D. (編集者)
要約:XII, 327 p.
text
言語:英語
出版事項: Totowa, NJ : Humana Press : Imprint: Humana, 2004.
版:2nd ed. 2004.
シリーズ:Methods in Molecular Biology, 284
主題:
オンライン・アクセス:https://doi.org/10.1385/1592598161
フォーマット: 電子媒体 図書
目次:
  • Making Protein Immunoprecipitates
  • Signal Transduction Inhibitors in Cellular Function
  • Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets
  • A High-Throughput Mammalian Cell-Based Transient Transfection Assay
  • Determining Protein Half-Lives
  • Assaying Protein Kinase Activity
  • Comparative Phosphorylation Site Mapping From Gel-Derived Proteins Using a Multidimensional ES/MS-Based Approach
  • Studies of Calmodulin-Dependent Regulation
  • Measurement of Protein-DNA Interactions In Vivo by Chromatin Immunoprecipitation
  • Characterization of Protein-DNA Association In Vivo by Chromatin Immunoprecipitation
  • Nonradioactive Methods for Detecting Activation of Ras-Related Small G Proteins
  • Nucleocytoplasmic Glycosylation, O-GlcNAc
  • Techniques in Protein Methylation
  • Assaying Lipid Phosphate Phosphatase Activities
  • Assaying Phosphoinositide Phosphatases
  • Assaying Phospholipase A2 Activity
  • Measurement and Immunofluorescence of Cellular Phosphoinositides
  • Measuring Dynamic Changes in cAMP Using Fluorescence Resonance Energy Transfer
  • In Vivo Detection of Protein-Protein Interaction in Plant Cells Using BRET
  • Revealing Protein Dynamics by Photobleaching Techniques
  • Assaying Cytochrome c Translocation During Apoptosis.