Lipoprotein Protocols

التفاصيل البيبلوغرافية
مؤلف مشترك: SpringerLink (Online service)
مؤلفون آخرون: Ordovas, Jose M. (المحرر)
الملخص:XI, 284 p.
text
اللغة:الإنجليزية
منشور في: Totowa, NJ : Humana Press : Imprint: Humana, 1998.
الطبعة:1st ed. 1998.
سلاسل:Methods in Molecular Biology, 110
الموضوعات:
الوصول للمادة أونلاين:https://doi.org/10.1385/1592595820
التنسيق: الكتروني كتاب
جدول المحتويات:
  • DNA- and RNA-Based Methodolog
  • PCR Methodology Applied to Genetic Studies of Lipoprotein Metabolism and Atherosclerosis
  • Separation of Small-Size DNA Fragments Using Agarose Gel Electrophoresis
  • Quantification of mRNA by Polymerase Chain Reaction (PCR) Using an Internal Standard and a Nonradioactive Detection Method
  • Generation of Transgenic Mice
  • Preparation of Knockout Mice
  • Protein-Based Methodology
  • Fast Ultracentrifugation Methods for the Separation of Plasma Lipoproteins
  • Preparative Isolation of Plasma Lipoproteins Using Fast Protein Liquid Chromatography (FPLC)
  • Separation of Apolipoproteins by Polyacrylamide Gel Electrophoresis
  • Isolation and Purification of Serum Lipoprotein (a) Using Lectin Affinity
  • Electrophoretic Separation of LDL and HDL Subclasses
  • Measurement of Lipoprotein Particles
  • Methodological Approaches for Assessing Lipid and Protein Oxidation and Modification in Plasma and Isolated Lipoproteins
  • Separation and Quantitation of Phospholipid Classes by HPLC
  • Assays of Lecithin Cholesterol Acyltransferase (LCAT)
  • Determination of the Mass Concentration and the Activity of the Plasma Cholesteryl Ester Transfer Protein (CETP)
  • In Vitro Measurement of Lipoprotein and Hepatic Lipases
  • Cellular Assays for Lipoprotein Receptors
  • Measurements of Proteoglycan—Lipoprotein Interaction by Gel Mobility Shift Assay.