Adhesion Protein Protocols
| Coauteur: | |
|---|---|
| Andere auteurs: | , |
| Samenvatting: | XIII, 229 p. 4 illus. text |
| Taal: | Engels |
| Gepubliceerd in: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1999.
|
| Editie: | 1st ed. 1999. |
| Reeks: | Methods in Molecular Biology,
96 |
| Onderwerpen: | |
| Online toegang: | https://doi.org/10.1385/1592592589 |
| Formaat: | Elektronisch Boek |
Inhoudsopgave:
- Monoclonal Antibodies Specific for Leukocyte Adhesion Molecules
- Epitope Mapping
- Sequencing of Antibodies
- Purification Through Affinity Chromatography and Microsequencing
- Functional Analysis of Cell Adhesion Molecules
- Application of Polymerase Chain Reaction for the Discovery of New Adhesion Molecule Family Members
- Construction and Purification of Adhesion Molecule Immunoglobulin Chimeric Proteins
- Immunofluorescence of Cultured Cells
- Detection of Adhesion Molecules by Immunohistochemistry on Human and Murine Tissue Sections
- In situ Hybridization with RNA Probes
- Purification of Fibronectin from Human Plasma
- Adhesion to Matrix Proteins
- Leukocyte-Endothelial Monolayer Adhesion Assay (Static Conditions)
- Cell-Cell Adhesive Interactions in an In Vitro Flow Chamber
- Adhesion of Tumor Cells to Endothelium Under Static Conditions
- Adhesion of Tumor Cells Under Flow
- Platelet Adhesion to the Subendothelium Under Flow
- Leukocyte Transmigration Through Vascular Endothelium
- Cell Migration into a Wounded Area In Vitro
- Determination of the Endothelial Barrier Function In Vitro
- Induction of Intracellular Signals Through Binding of Adhesive Molecules
- Induction of Ca2+ Flux by Adhesion Molecules in Lymphocytes
- Integrin-Mediated Stimulation of Tyrosine Phosphorylation in Lymphoid Cells
- The Identification of Signaling Molecules by the Yeast Two-Hybrid System.