Adhesion Protein Protocols

التفاصيل البيبلوغرافية
مؤلف مشترك: SpringerLink (Online service)
مؤلفون آخرون: Dejana, Elisabetta (المحرر), Corada, Monica (المحرر)
الملخص:XIII, 229 p. 4 illus.
text
اللغة:الإنجليزية
منشور في: Totowa, NJ : Humana Press : Imprint: Humana, 1999.
الطبعة:1st ed. 1999.
سلاسل:Methods in Molecular Biology, 96
الموضوعات:
الوصول للمادة أونلاين:https://doi.org/10.1385/1592592589
التنسيق: الكتروني كتاب
جدول المحتويات:
  • Monoclonal Antibodies Specific for Leukocyte Adhesion Molecules
  • Epitope Mapping
  • Sequencing of Antibodies
  • Purification Through Affinity Chromatography and Microsequencing
  • Functional Analysis of Cell Adhesion Molecules
  • Application of Polymerase Chain Reaction for the Discovery of New Adhesion Molecule Family Members
  • Construction and Purification of Adhesion Molecule Immunoglobulin Chimeric Proteins
  • Immunofluorescence of Cultured Cells
  • Detection of Adhesion Molecules by Immunohistochemistry on Human and Murine Tissue Sections
  • In situ Hybridization with RNA Probes
  • Purification of Fibronectin from Human Plasma
  • Adhesion to Matrix Proteins
  • Leukocyte-Endothelial Monolayer Adhesion Assay (Static Conditions)
  • Cell-Cell Adhesive Interactions in an In Vitro Flow Chamber
  • Adhesion of Tumor Cells to Endothelium Under Static Conditions
  • Adhesion of Tumor Cells Under Flow
  • Platelet Adhesion to the Subendothelium Under Flow
  • Leukocyte Transmigration Through Vascular Endothelium
  • Cell Migration into a Wounded Area In Vitro
  • Determination of the Endothelial Barrier Function In Vitro
  • Induction of Intracellular Signals Through Binding of Adhesive Molecules
  • Induction of Ca2+ Flux by Adhesion Molecules in Lymphocytes
  • Integrin-Mediated Stimulation of Tyrosine Phosphorylation in Lymphoid Cells
  • The Identification of Signaling Molecules by the Yeast Two-Hybrid System.