Adhesion Protein Protocols
| Corporate Author: | |
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| Other Authors: | , |
| Summary: | XIII, 229 p. 4 illus. text |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1999.
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| Edition: | 1st ed. 1999. |
| Series: | Methods in Molecular Biology,
96 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/1592592589 |
| Format: | Electronic Book |
Table of Contents:
- Monoclonal Antibodies Specific for Leukocyte Adhesion Molecules
- Epitope Mapping
- Sequencing of Antibodies
- Purification Through Affinity Chromatography and Microsequencing
- Functional Analysis of Cell Adhesion Molecules
- Application of Polymerase Chain Reaction for the Discovery of New Adhesion Molecule Family Members
- Construction and Purification of Adhesion Molecule Immunoglobulin Chimeric Proteins
- Immunofluorescence of Cultured Cells
- Detection of Adhesion Molecules by Immunohistochemistry on Human and Murine Tissue Sections
- In situ Hybridization with RNA Probes
- Purification of Fibronectin from Human Plasma
- Adhesion to Matrix Proteins
- Leukocyte-Endothelial Monolayer Adhesion Assay (Static Conditions)
- Cell-Cell Adhesive Interactions in an In Vitro Flow Chamber
- Adhesion of Tumor Cells to Endothelium Under Static Conditions
- Adhesion of Tumor Cells Under Flow
- Platelet Adhesion to the Subendothelium Under Flow
- Leukocyte Transmigration Through Vascular Endothelium
- Cell Migration into a Wounded Area In Vitro
- Determination of the Endothelial Barrier Function In Vitro
- Induction of Intracellular Signals Through Binding of Adhesive Molecules
- Induction of Ca2+ Flux by Adhesion Molecules in Lymphocytes
- Integrin-Mediated Stimulation of Tyrosine Phosphorylation in Lymphoid Cells
- The Identification of Signaling Molecules by the Yeast Two-Hybrid System.