Fundamental Approaches to Screen Abnormalities in Drosophila
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| Weitere Verfasser: | |
| Zusammenfassung: | VII, 265 p. 116 illus., 111 illus. in color. text |
| Sprache: | Englisch |
| Veröffentlicht: |
New York, NY :
Springer US : Imprint: Springer,
2020.
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| Ausgabe: | 1st ed. 2020. |
| Schriftenreihe: | Springer Protocols Handbooks,
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| Schlagworte: | |
| Online-Zugang: | https://doi.org/10.1007/978-1-4939-9756-5 |
| Format: | Elektronisch E-Book |
Inhaltsangabe:
- Chapter 1. Preparation of normal food and modified food for Drosophila
- Chapter 2. Protocol for dissection of various tissues (gut, imaginal disc, fat body, ovary, testes) in larva and adult fly
- Chapter 3. Biochemical estimation of various metabolites (protein, lipid, carbohydrate, trehalose, cholesterol)
- Chapter 4. Detection of fat within the Drosophila body
- Chapter 5. Estimation of reactive oxygen species (ROS) production in Drosophila cells
- Chapter 6. Methods to check the metabolic activity of fly
- Chapter 7. Detection of DNA damage in larva and adult stage of Drosophila by Fluorescent microscopy
- Chapter 8. Imaging of larva/adult gut damage using Bright field microscopy
- Chapter 9. A comet assay to detect severity of DNA damage
- Chapter 10. Checking the abnormality in hemolymph by Giemsa stain
- Chapter 11. Analysis of channel proteins
- Chapter 12. Various behavioral assays in larva and adult Drosophila
- Chapter 13. Detection of various metals within the tissue by Energy dispersive X-ray (EDX) through SEM and FTIR
- Chapter 14. Imaging of various sections of fly through Scanning Electron Microscopy (SEM)
- Chapter 15. Preparation of thin sections and fixation of various tissues of Drosophila for examination by transmission electron microscopy (TEM)
- Chapter 16. Flow cytometric analysis of Drosophila cells.