Fundamental Approaches to Screen Abnormalities in Drosophila

書誌詳細
団体著者:	SpringerLink (Online service)
その他の著者:	Mishra, Monalisa (編集者)
要約:	VII, 265 p. 116 illus., 111 illus. in color. text
言語:	英語
出版事項:	New York, NY : Springer US : Imprint: Springer, 2020.
版:	1st ed. 2020.
シリーズ:	Springer Protocols Handbooks,
主題:	Developmental biology. Psychobiology. Human behavior. Biology > Technique. Bioinformatics. Biophysics. Developmental Biology and Stem Cells. Behavioral Neuroscience. Experimental Organisms. Computational and Systems Biology. Bioanalysis and Bioimaging.
オンライン･アクセス:	https://doi.org/10.1007/978-1-4939-9756-5
フォーマット:	電子媒体図書

目次:

Chapter 1. Preparation of normal food and modified food for Drosophila
Chapter 2. Protocol for dissection of various tissues (gut, imaginal disc, fat body, ovary, testes) in larva and adult fly
Chapter 3. Biochemical estimation of various metabolites (protein, lipid, carbohydrate, trehalose, cholesterol)
Chapter 4. Detection of fat within the Drosophila body
Chapter 5. Estimation of reactive oxygen species (ROS) production in Drosophila cells
Chapter 6. Methods to check the metabolic activity of fly
Chapter 7. Detection of DNA damage in larva and adult stage of Drosophila by Fluorescent microscopy
Chapter 8. Imaging of larva/adult gut damage using Bright field microscopy
Chapter 9. A comet assay to detect severity of DNA damage
Chapter 10. Checking the abnormality in hemolymph by Giemsa stain
Chapter 11. Analysis of channel proteins
Chapter 12. Various behavioral assays in larva and adult Drosophila
Chapter 13. Detection of various metals within the tissue by Energy dispersive X-ray (EDX) through SEM and FTIR
Chapter 14. Imaging of various sections of fly through Scanning Electron Microscopy (SEM)
Chapter 15. Preparation of thin sections and fixation of various tissues of Drosophila for examination by transmission electron microscopy (TEM)
Chapter 16. Flow cytometric analysis of Drosophila cells.

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