Ancient DNA Methods and Protocols /
| Körperschaft: | |
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| Weitere Verfasser: | , , , , , |
| Zusammenfassung: | X, 216 p. 15 illus., 5 illus. in color. text |
| Sprache: | Englisch |
| Veröffentlicht: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Ausgabe: | 2nd ed. 2019. |
| Schriftenreihe: | Methods in Molecular Biology,
1963 |
| Schlagworte: | |
| Online-Zugang: | https://doi.org/10.1007/978-1-4939-9176-1 |
| Format: | Elektronisch E-Book |
Inhaltsangabe:
- Setting Up an Ancient DNA Laboratory
- Pretreatment: Removing DNA Contamination from Ancient Bones and Teeth Using Sodium Hypochlorite and Phosphate
- Pretreatment: Improving Endogenous Ancient DNA Yields Using a Simple Enzymatic Pre-Digestion Step
- Extraction of Highly Degraded DNA from Ancient Bones and Teeth
- Sampling and Extraction of Ancient DNA from Sediments
- Extraction of Ancient DNA from Plant Remains
- DNA Extraction from Keratin and Chitin
- Double-Stranded Library Preparation for Ancient and Other Degraded Samples
- A Method for Single-Stranded Ancient DNA Library Preparation
- Sequencing Library Preparation from Degraded Samples for Non-Illumina Sequencing Platforms
- Whole-Genome Capture of Ancient DNA Using Homemade Baits
- Generating RNA Baits for Capture-Based Enrichment
- Hybridization Capture of Ancient DNA Using RNA Baits
- Application of Solid-State Capture for the Retrieval of Small-to-Medium Sized Target Loci from Ancient DNA
- Targeted PCR Amplification and Multiplex Sequencing of Ancient DNA for SNP Analysis
- Targeted Amplification and Sequencing of Ancient Environmental and Sedimentary DNA
- Authentication and Assessment of Contamination in Ancient DNA
- Assembly of Ancient Mitochondrial Genomes without a Closely Related Reference Sequence.