Synthetic Biology Methods and Protocols /
| Corporate Author: | |
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| Other Authors: | |
| Summary: | XV, 510 p. 109 illus., 82 illus. in color. text |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2018.
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| Edition: | 1st ed. 2018. |
| Series: | Methods in Molecular Biology,
1772 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-4939-7795-6 |
| Format: | Electronic Book |
Table of Contents:
- Re-Factoring Glycolytic Genes for Targeted Engineering of Catabolism in Gram-Negative Bacteria
- Negative Regulation Gene Circuits for Efflux Pump Control
- Methods for Generating Microbial Co-Cultures that Grow in the Absence of Fixed Carbon or Nitrogen
- Synthetic Biology with an All E. coli TXTL System: Quantitative Characterization of Regulatory Elements and Gene Circuits
- Transformation of an Exotic Yeast Species into a Platform Organism: A Case Study for Engineering Glycolipid Production in the Yeast Starmerella bombicola
- Sortase A-Assisted Metabolic Enzyme Ligation in Escherichia coli for Enhancing Metabolic Flux
- Construction of CRISPR Libraries for Functional Screening
- Targeted Genome Editing Using DNA-Free RNA-Guided Cas9 Ribonucleoprotein for CHO Cell Engineering
- Dynamic Management of Codon Compression for Saturation Mutagenesis
- Designed Transcriptional Regulation in Mammalian Cells Based on TALE- and CRISPR/dCas9
- Combinatorial Evolution of DNA with RECODE
- Genome Editing in Penicillium chrysogenum Using Cas9 Ribonucleoprotein Particles
- Targeted Gene Knockout Using Nuclease-Assisted Vector Integration: Hemi- and Homozygous Deletion of JAG1
- Programming Biomaterial Interactions Using Engineered Living Cells
- Rapid Control of Genome Editing in Human Cells by Chemical-Inducible CRISPR-Cas Systems
- Production of a Human Cell Line with a Plant Chromosome
- Bacterial Genome Editing with CRISPR-Cas9: Taking Clostridium beijerinckii as an Example
- CRISPR-Cas9-Mediated Genome Editing and Transcriptional Control in Yarrowia lipolytica
- Construction and Integration of a Synthetic MicroRNA Cluster for Multiplex RNA Interference in Mammalian Cells
- Bio-Algorithmic Workflows for Standardized Synthetic Biology Constructs
- Engineering Genomes with Genotype Specification Language
- Automated Visualization of Genetic Designs Using DNAplotlib
- DNA-Based Analog Computing
- DNA Assemblywith the DATEL Method
- EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology
- Designing and Assembling Plasmids for Construction of Escherichia coli Biosensor for Vibrio cholerae Detection
- A Rapid Combinatorial Approach to Assembling Synthetic Prokaryotic and Eukaryotic Protein Expression Vectors
- Standardized Cloning and Curing of Plasmids
- STAR: A Simple TAL Effector Assembly Reaction Using Isothermal Assembly.