Proteomics Methods and Protocols /

Dettagli Bibliografici
Ente Autore: SpringerLink (Online service)
Altri autori: Comai, Lucio (Redattore), Katz, Jonathan E. (Redattore), Mallick, Parag (Redattore)
Riassunto:XII, 371 p. 64 illus., 48 illus. in color.
text
Lingua:inglese
Pubblicazione: New York, NY : Springer New York : Imprint: Humana, 2017.
Edizione:1st ed. 2017.
Serie:Methods in Molecular Biology, 1550
Soggetti:
Accesso online:https://doi.org/10.1007/978-1-4939-6747-6
Natura: Elettronico Libro
Sommario:
  • A Robust Protocol for Protein Extraction and Digestion
  • Improving Proteome Coverage and Sample Recovery with Enhanced FASP (Efasp) For Quantitative Proteomic Experiments
  • Proteome Characterization of a Chromatin Locus using the Proteomics of Isolated Chromatin Segments Approach
  • Profiling Cell Lines Nuclear Sub-Proteome
  • Optimized Enrichment of Phosphoproteomes by Fe-IMAC Column Chromatography
  • Full Membrane Protein Coverage Digestion And Quantitative Bottom-Up Mass Spectrometry Proteomics
  • Hydrophilic Strong Anion Exchange (Hsax) Chromatography Enables Deep Fractionation Of Tissue Proteomes
  • High Ph Reversed-Phase Micro-Columns for Simple, Sensitive and Efficient Fractionation of Proteome and (TMT-Labeled) Phosphoproteome Digests
  • Multi-Lectin Affinity Chromatography for Separation, Identification, and Quantitation of Intact Protein Glycoforms in Complex Biological Mixtures
  • Parallel Exploration of Interaction Space by Bioid and Affinity Purification Coupled to Mass Spectrometry
  • LUMIER: A Discovery Tool for Mammalian Protein Interaction Networks
  • Dual Color, Multiplex Analysis of Protein Microarrays for Precision Medicine
  • Quantitative Proteomics Using SILAC
  • Relative Protein Quantification using Tandem Mass Tag Mass Spectrometry
  • Pathway-Informed Discovery and Targeted Proteomic Workflows Using Mass Spectrometry
  • Generation of High Quality SWATH Acquisition Data for Label-Free Quantitative Proteomics Studies Using Tripletof® Mass Spectrometers
  • Annotating Mutational Effects on Proteins and Protein Interactions: Designing Novel and Revisiting Existing Protocols
  • Protein Micropatterning Assay - Quantitative Analysis of Protein-Protein Interactions
  • Designing Successful Proteomics Experiments
  • Automated SWATH Data Analysis using Targeted Extraction of Ion Chromatograms
  • Virtualization of Legacy Instrumentation Control Computers for Improved Reliability, Operational Life, and Management
  • Statistical Assessment of QC Metrics on Raw LC-MS/MS Data
  • Data Conversion with proteoWizard msConvert.