Animal Endo-SiRNAs Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Werner, Andreas (Editor)
Summary:X, 182 p. 36 illus., 24 illus. in color.
text
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2014.
Edition:1st ed. 2014.
Series:Methods in Molecular Biology, 1173
Subjects:
Online Access:https://doi.org/10.1007/978-1-4939-0931-5
Format: Electronic Book
Table of Contents:
  • Targeted Small Non-coding RNA Directed Gene Activation in Human Cells
  • Isolation of Chromatoid Dodies from Mouse Testis as a Rich Source of Short RNAs
  • Generation of Endo-siRNAs in Xenopus laevis Oocytes
  • Analysis of Endo-siRNAs in Drosophila
  • Methods for Studying the Biological Consequences of Endo-siRNA Deficiency in Drosophila melanogaster
  • Small RNA Library Cloning Procedure for Deep Sequencing of Specific Endogenous siRNA Classes in Caenorhabditis elegans
  • Assays for Direct and Indirect Effects of C. elegans Endo-siRNAs
  • Extraction and Non-radioactive Detection of Small RNA Molecules
  • p19 Mediated Enrichment and Detection of siRNAs
  • Detection of Small non-coding RNAs by in situ Hybridization using Probes of 2’-O-Methyl RNA + LNA
  • Enhanced Detection of Small RNAs using a non-Radioactive Approach
  • Computing siRNA and piRNA Overlap Signatures
  • Isolation of Small Interfering RNAs using Viral Suppressors of RNA Interference
  • Computational Analysis, Biochemical Purification and Detection of tRNA-derived Small RNA Fragments
  • Differential DNA Methylation Patterns in Endo-siRNAs Mediated Silencing of LINE-1 Retrotransposons.