Oncogene-Induced Senescence Methods and Protocols /

Bibliographische Detailangaben
Körperschaft: SpringerLink (Online service)
Weitere Verfasser: Gorgoulis, Vassilis G. (HerausgeberIn), Cavinato, Maria (HerausgeberIn), Evangelou, Konstantinos (HerausgeberIn)
Zusammenfassung:XIII, 363 p. 57 illus., 54 illus. in color.
text
Sprache:Englisch
Veröffentlicht: New York, NY : Springer US : Imprint: Humana, 2025.
Ausgabe:1st ed. 2025.
Schriftenreihe:Methods in Molecular Biology, 2906
Schlagworte:
Online-Zugang:https://doi.org/10.1007/978-1-0716-4426-3
Format: Elektronisch E-Book
Inhaltsangabe:
  • In Situ and In Silico Methods for Senescence Identification in Human Liver Diseases
  • Current Methodologies to Assess Cellular Senescence in Cancer
  • Selective Sorting of Senescent Cell Subpopulations Compatible with Downstream Genomics Pipelines
  • Detection and Characterization of Senescent Cells with Flow Cytometry
  • Detecting Senescence in T Cells by Flow Cytometry Using the SA-β-galactosidase Assay
  • The DNA Damage Response as an Auxiliary Indicator of Senescence in Cancer: A User-Friendly Toolkit of Markers and Detection Methods
  • Precise Mapping of Physiological DSBs Using In-Suspension Break Labelling In Situ and Sequencing (sBLISS)
  • Detecting Telomeric DNA Damage by Immuno-Telo FISH
  • Combined In Situ Transcriptomic and Immunofluorescence to Assess the Effects on DNA Damage and Innate Immune Responses Induced by Bacterial Genotoxin in In Vivo Models
  • Two-Replication Round Telomere Strand-Specific Chromosome Orientation Fluorescent In Situ Hybridization
  • Identification of Oncogene-Induced Senescence-Associated MicroRNAs
  • Global Proteomic Profiling to Unravel Lipofuscin’s Protein Repertoire
  • Electron Microscopy to Visualize and Quantify Mitochondrial Structure and Organellar Interactions in Cultured Cells during Senescence
  • Isolation and Characterization of EVs Containing Mitochondria from OIS Cells
  • Isolation and Characterization of Endothelial Colony Forming Cells (ECFC): Studying Endothelial Senescence for Translational Studies and for Personalized Medicine
  • In Vitro Modeling of Vascular Senescence
  • Studying Cellular Senescence Using the Model-Organism Drosophila melanogaster
  • C. elegans as a Screening Platform for Anti-Aging Compounds
  • Natural Product-Derived Senotherapeutics: Extraction and Biological Evaluation Techniques.