Rhinovirus Methods and Protocols /
| 団体著者: | |
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| その他の著者: | |
| 要約: | X, 194 p. 48 illus., 43 illus. in color. text |
| 言語: | 英語 |
| 出版事項: |
New York, NY :
Springer US : Imprint: Humana,
2025.
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| 版: | 1st ed. 2025. |
| シリーズ: | Methods in Molecular Biology,
2903 |
| 主題: | |
| オンライン･アクセス: | https://doi.org/10.1007/978-1-0716-4410-2 |
| フォーマット: | 電子媒体 図書 |
目次:
- Detection of virus in clinical samples by PCR
- Laboratory protocol for propagation and purification of rhinovirus A and B suitable for in vitro and in vivo infection
- Method to generate high-titre rhinovirus C for in vitro and in vivo studies
- Quantification of infectious rhinovirus A and B by plaque assay
- Quantifying Rhinovirus Genomic and Negative-Strand RNA by RT-PCR
- Determination of binding and endocytosis of rhinovirus by flow cytometry
- Assessment of viral replication in rhinovirus-infected airway epithelial cells
- Analysis of adherence junctions in rhinovirus-infected airway epithelial cells
- Culturing of airway stem cells obtained from COPD and asthma patients to assess the effects of rhinovirus infection
- Investigation of Differentiated Nasal Epithelial Responses to Infection with Clinical Isolates of Rhinovirus A and C
- In vivo model to assess the nasal infection by minor group rhinovirus
- Identification and function of type 2 innate lymphoid cells in rhinovirus-infected mouse model of asthma
- Assessment of rhinovirus infection in a mouse model of COPD
- Infection of immature mice with rhinovirus for the study of asthma pathogenesis
- In vitro model to test therapeutic agents against rhinovirus.