Phage Display Methods and Protocols /
| Autor Corporativo: | |
|---|---|
| Outros Autores: | , |
| Resumo: | XV, 605 p. 83 illus., 69 illus. in color. text |
| Idioma: | inglês |
| Publicado em: |
New York, NY :
Springer US : Imprint: Humana,
2023.
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| Edição: | 2nd ed. 2023. |
| coleção: | Methods in Molecular Biology,
2702 |
| Assuntos: | |
| Acesso em linha: | https://doi.org/10.1007/978-1-0716-3381-6 |
| Formato: | Recurso Eletrônico Livro |
Sumário:
- Antibody phage display
- Construction of human immune and naive scFv Phage Display libraries
- Construction of Naïve and Immune Human Fab Phage Display Library
- Construction of synthetic antibody phage display libraries
- Construction of chicken and ostrich antibody libraries
- Construction of Rabbit Immune Antibody Libraries
- Isolation and characterization of single-domain antibodies from immune phage display libraries
- Phagekines: directed evolution and characterization of functional cytokines displayed on phages
- Efficient Cloning of Inserts for Phage Display by Golden Gate Assembly
- Construction of an ultra-large phage display library by Kunkel mutagenesis and rolling circle amplification
- Construction of semi-synthetic shark vNAR yeast surface display antibody libraries
- Antibody selection via Phage Display in microtiter plates
- Antibody selection in solution using magnetic beads
- Streptavidin Coated Solid Phase Extraction (SPE) TipsFor Antibody Phage Display Biopanning
- Magnetic Nanoparticle-Based Semi-Automated Panning for High-throughput Antibody Selection
- Antibody selection on cells targeting membrane proteinsTargeting Intracellular Antigens With pMHC-binding Antibodies: A Phage Display Approach
- Antibody Isolation from a Human Synthetic Libraries of Single-chain Antibodies and analysis using NGS
- Selection of affibody affinity proteins from phagemid libraries
- Antibody affinity and stability maturation by error-prone PCR
- Antibody batch cloning
- Deep Mining of Complex Antibody Phage Pools
- High-throughput IgG reformatting and expression using hybrid secretion signals and InTag positive selection technology
- Validation and the Determination of Antibody Bioactivity Using MILKSHAKE and Sundae Protocols
- Mapping polyclonal antibody responses to infection using next generation phage display
- Applications of high-throughput DNA sequencing to single-domain antibodydiscovery and engineering
- Biomarker discovery by ORFeome Phage Display
- Mapping Epitopes by Phage Display
- Epivolve: A Protocol for Site-directed Antibodies.