Transposable Elements Methods and Protocols /

التفاصيل البيبلوغرافية
مؤلف مشترك: SpringerLink (Online service)
مؤلفون آخرون: Branco, Miguel R. (المحرر), de Mendoza Soler, Alexandre (المحرر)
الملخص:XII, 382 p. 65 illus., 60 illus. in color.
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اللغة:الإنجليزية
منشور في: New York, NY : Springer US : Imprint: Humana, 2023.
الطبعة:1st ed. 2023.
سلاسل:Methods in Molecular Biology, 2607
الموضوعات:
الوصول للمادة أونلاين:https://doi.org/10.1007/978-1-0716-2883-6
التنسيق: الكتروني كتاب
جدول المحتويات:
  • An overview of best practices for transposable element identification, classification and annotation in eukaryotic genomes
  • Assembly-free annotation and quantification of transposable elements with dnaPipeTE
  • Best practice for the identification of horizontally transferred transposons
  • Genotyping of transposable elements insertions segregating in the human populations using short-read re-alignments
  • A Pangenome approach to detect and genotype TE insertion polymorphisms.0 Experimental validation of transposable element insertions using the Polymerase Chain Reaction (PCR)
  • Quantification of LINE-1 RNA expression from bulk RNA-seq using L1EM
  • Genome-wide profiling of L1 DNA methylation by bs-ATLAS-seq
  • Nanopore epigenomic analysis of transposable element DNA modifications
  • Targeted resequencing and methylation analysis of L1 elements by nanopore sequencing
  • Inferring protein-DNA binding profiles at interspersed repeats using HiChIP and PatChER
  • Affinity-based Interactome Analysis of Endogenous LINE-1 Macromolecules
  • LINE-1 retrotransposition assays in embryonic stem cells
  • Detecting somatic transposable element insertions in Drosophila tissues
  • Precise and scarless insertion of transposable elements by Cas9-mediated genome engineering
  • Epigenetic editing of transposable and repetitive elements
  • Using CRISPR to investigate the regulatory activity of transposable elements. .