Archaea Methods and Protocols /

Detaylı Bibliyografya
Müşterek Yazar: SpringerLink (Online service)
Diğer Yazarlar: Ferreira-Cerca, Sébastien (Editör)
Özet:XIV, 549 p. 98 illus., 88 illus. in color.
text
Dil:İngilizce
Baskı/Yayın Bilgisi: New York, NY : Springer US : Imprint: Humana, 2022.
Edisyon:1st ed. 2022.
Seri Bilgileri:Methods in Molecular Biology, 2522
Konular:
Online Erişim:https://doi.org/10.1007/978-1-0716-2445-6
Materyal Türü: Elektronik Kitap
İçindekiler:
  • Archaea: a goldmine for molecular biologists and evolutionists
  • Progress and challenges in archaeal genetic manipulation
  • Genetic manipulation of Haloferax species
  • CRISPR interference as a tool to Repress Gene Expression in Haloferax volcanii
  • Transformation Techniques for the Anaerobic Hyperthermophile Thermococcus kodakarensis
  • Genetic Methods and Construction of Chromosomal Mutations in Methanogenic Archaea
  • An interdomain conjugation protocol for plasmid-DNA transfer into Methanothermobacter thermautotrophicus ΔH
  • Methods for Markerless Gene Deletion and Plasmid-Based Expression in Sulfolobus acidocaldarius
  • A Rapid Targeted Gene Inactivation Approach in Sulfolobus islandicus
  • Transposon Insertion Mutagenesis in hyperthermophilic crenarchaeon Sulfolobus islandicus
  • Reprogramming CRISPR-mediated RNA interference for silencing of essential genes in Sulfolobales
  • Progress and challenges in archaeal molecular biology
  • ChIP-seq Occupancy Mapping of the Archaeal Transcription Machinery
  • Conversion of ribosome-protected mRNA to DNA and deep sequencing for ribosome profiling in Haloferax volcanii
  • Small RNA-sequencing library preparation for the halophilic archaeon Haloferax volcanii
  • Quantitative Mass Spectrometry by SILAC in Haloferax volcanii
  • Proteome turnover analysis in Haloferax volcanii by a heavy isotope multilabelling approach
  • Proteomic sample preparation and data analysis in line with the Archaeal Proteome
  • In vivo protein crosslinking and co-immunoprecipitation in Haloferax volcanii
  • Proteolytic activity assays in haloarchaea
  • Carotenoids from Haloarchaea: Extraction, fractionation and characterization
  • Metal nanoparticles biosynthesis using the halophilic archaeon Haloferax volcanii
  • Simplified enzymatic synthesis of 2-keto-3-deoxy-D-gluconate from D-gluconate using the gluconate dehydratase from Thermoproteus tenax
  • Progress and Challenges in Archaeal Cellular Biology Analysis
  • Methods to Analyze Motility in Eury- And Crenarchaea
  • Immersed Liquid Biofilm and Honeycomb Pattern Formations in Haloferax volcanii
  • Cost-effective and Versatile Analysis of Archaeal Surface Adhesion Under Shaking and Standing Conditions
  • Cell Adhesion and Biofilm Formation Analysis
  • BrdU incorporation and labelling of nascent DNA to Investigate Archaeal Replication using super-resolution imaging
  • Isolation, Purification, and Characterization of Membrane Vesicles from Haloarchaea
  • Archaeal Viruses: Production of Virus Particles and Vesicle-Like Viruses and Purification Using Asymmetrical Flow Field-Flow Fractionation
  • Progress and Challenges in Studying the Ecophysiology of Archaea
  • Reconstruction of Archaeal Genomes Assembly from Short-Read Metagenomes
  • Outreach: Accessible and Insightful Scientific Learning Experiences Using the Microorganism Haloferax volcanii.