Biophysics of Membrane Proteins Methods and Protocols /

Opis bibliograficzny
Korporacja: SpringerLink (Online service)
Kolejni autorzy: Postis, Vincent L. G. (Redaktor), Goldman, Adrian (Redaktor)
Streszczenie:XI, 336 p. 73 illus., 61 illus. in color.
text
Język:angielski
Wydane: New York, NY : Springer US : Imprint: Humana, 2020.
Wydanie:1st ed. 2020.
Seria:Methods in Molecular Biology, 2168
Hasła przedmiotowe:
Dostęp online:https://doi.org/10.1007/978-1-0716-0724-4
Format: Elektroniczne E-book
Spis treści:
  • Membrane Protein Production and Purification from Escherichia coli and Sf9 Insect Cells
  • Quantifying the Interaction of Phosphite with ABC Transporters: MicroScale Thermophoresis and a Novel His-tag Labeling Approach
  • Rationale for the Quantitative Reconstitution of Membrane Proteins into Proteoliposomes
  • Functional Characterization of SLC transporters using Solid-Supported-Membranes
  • Thermostability Assays Provide a Generic and Versatile Tool for Studying the Functional and Structural Properties of Membrane Proteins in Detergents
  • Direct monitoring of GPCR reconstitution and ligand binding activity by Plasmon Waveguide Resonance
  • Examining Membrane Proteins by Neutron Scattering
  • Solution X-ray Scattering for Membrane Protein
  • Interpreting SAXS/WAXS Data with Explicit-Solvent Simulations: A Practical Guide
  • Determining the Free Energies of Outer Membrane Proteins in Lipid Bilayers
  • Interrogating Membrane Protein Structure and Lipid Interactions by Native Mass Spectrometry
  • Determination of the Molecular Mass of Membrane Proteins Using Size Exclusion Chromatography with Multi-angle Laser Light Scattering (SEC-MALLS)
  • Dynamics of Membrane Proteins Monitored by Single-molecule Fluorescence across Multiple Time Scale
  • Short-Range Distances Measurement by Transition Metal Ion FRET
  • PELDOR/DEER – An Electron Paramagnetic Resonance Method to Study Membrane Proteins in Lipid Bilayers. .