MERS Coronavirus Methods and Protocols /
| Autor Corporativo: | |
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| Outros Autores: | |
| Resumo: | XI, 224 p. 40 illus., 27 illus. in color. text  | 
| Idioma: | inglês | 
| Publicado em: | 
        New York, NY :
          Springer US : Imprint: Humana,
    
        2020.
     | 
| Edição: | 1st ed. 2020. | 
| coleção: | Methods in Molecular Biology,
              2099             | 
| Assuntos: | |
| Acesso em linha: | https://doi.org/10.1007/978-1-0716-0211-9 | 
| Formato: | Recurso Eletrônico Livro | 
                Sumário: 
            
                  - Studying Evolutionary Adaptation of MERS-CoV
 - Evaluating MERS-CoV Entry Pathways
 - Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing
 - Crystallization and Structural Determination of the Receptor-binding Domain of MERS-CoV Spike Glycoprotein
 - Bacterial Artificial Chromosome-based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV
 - Deducing the Crystal Structure of MERS-CoV Helicase
 - Antigen Capture Enzyme-linked Immunosorbent Assay for Detecting Middle East Respiratory Syndrome Coronavirus in Humans
 - Quantification of the Middle East Respiratory Syndrome-Coronavirus RNA in Tissues by Quantitative Real-Time RT-PCR
 - Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay
 - Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera
 - Qualitative and Quantitative Determination of MERS-CoV S1-specific Antibodies using ELISA
 - Genetically Engineering a Susceptible Mouse Model for MERS-CoV-Induced Acute Respiratory Distress Syndrome
 - Development of a Mouse Adapted MERS Coronavirus
 - Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection
 - Evaluation of Activation and Inflammatory Activity of Myeloid Cells DuringPathogenic Human Coronavirus Infection
 - Histopathologic Evaluation and Scoring of Viral Lung Infection. .