Water-Soluble Polysaccharides of Alfalfa (Medicago sativa (Fabaceae)) of Flora of Krasnoyarsk Krai
| Parent link: | Russian Journal of Bioorganic Chemistry Vol. 44, iss. 7.— 2018.— [P. 854-859] |
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| Other Authors: | , , , , |
| Summary: | Title screen Alfalfa is a very high-quality perennial leguminous grass with a long history of cultivation and application in traditional medicine in Russia. In addition, alfalfa is a valuable forage representative of the legume family, its acreage in Russia reaches 2.3-2.5 million ha. The polysaccharide complex (PSC) was isolated from the aerial part of alfalfa by aqueous extraction (pH = 9) and characterized by physicochemical methods. The content of uronic acids was 13.1 ± 0.9%, the quantitative content of impurity substances such as protein was 4.25 ± 0.28%, nucleic acids 0.0056 ± 0.0003%, and mineral impurities (ash content) 3.45 ± 0.18%. The PSC was studied by IR spectroscopy. The monomeric composition of PSC was characterized by chromatography-mass spectrometry (major monosugars arabinose, glucose and galacturonic acid). The molecular weight distribution of PSC was studied by the high-pressure exclusion chromatography method. Data demonstrated that the polysaccharide complex of the aerial part of alfalfa consists of three components with molecular weights of 1100 ± 60, 16 ± 2 and 7 ± 1 kDa. The fact that PSC is a mixture of seven polysaccharides was demonstrated by the column ion-exchange chromatography method. Режим доступа: по договору с организацией-держателем ресурса |
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2018
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| Online Access: | https://doi.org/10.1134/S1068162018070105 |
| Format: | Electronic Book Chapter |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=661322 |