“Solvent/non-solvent” treatment as a method for non-covalent immobilization of gelatin on the surface of poly(l-lactic acid) electrospun scaffolds

Bibliographic Details
Parent link:Colloids and Surfaces B: Biointerfaces
Vol. 177.— 2019.— [P. 137-140]
Corporate Authors: Национальный исследовательский Томский политехнический университет Инженерная школа новых производственных технологий Научно-образовательный центр Н. М. Кижнера, Национальный исследовательский Томский политехнический университет Инженерная школа ядерных технологий Научно-образовательный центр Б. П. Вейнберга
Other Authors: Goreninsky S. I. Semen Igorevich, Gulyaev R. O. Roman Olegovich, Stankevich K. S. Ksenia Sergeevnahttps://online.sberbank.ru/CSAFront/index.do, Danilenko N. V. Nadezhda Viktorovna, Bolbasov E. N. Evgeny Nikolaevich, Golovkin A. S. Aleksey Sergeevich, Mishanin A. I. Aleksandr, Filimonov V. D. Viktor Dmitrievich, Tverdokhlebov S. I. Sergei Ivanovich
Summary:Title screen
In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0°, enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07?±?0.01?mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.
Режим доступа: по договору с организацией-держателем ресурса
Published: 2019
Subjects:
Online Access:https://doi.org/10.1016/j.colsurfb.2019.01.060
Format: Electronic Book Chapter
KOHA link:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=659633