Quantitative determination of new anti-inflammatory drug indomenthyl and its metabolite in rabbit plasma by HPLC-MS/MS; Analytical Methods; Vol. 13, iss. 41

Quantitative determination of new anti-inflammatory drug indomenthyl and its metabolite in rabbit plasma by HPLC-MS/MS

Библиографические подробности
Источник:Analytical Methods
Vol. 13, iss. 41.— 2021.— [9 p.]
Автор-организация: Национальный исследовательский Томский политехнический университет Инженерная школа природных ресурсов Отделение химической инженерии
Другие авторы: Leonov K. A. Klim Andreevich, Bykova A. V. Arina Vladimirovna, Bykov V. V. Vladimir Valerjevich, Vishenkova D. A. Dariya Aleksandrovna, Lipskikh O. I. Olga Ivanovna, Dorozhko E. V. Elena Vladimirovna, Pavlovsky V. I. Viktor Ivanovich, Vengerovsky A. I. Aleksandr Isaakovich, Udut V. V. Vladimir Vasiljevich
Примечания:Title screen
Background: Indomenthyl is an innovative anti-inflammatory drug with a high analgesic activity. Indomenthyl releases indomethacin under the influence of neutrophil esterases in the inflammation focus. Methodology/results: This research is aimed at developing a highly sensitive method for the quantitative determination of indomenthyl and its active metabolite indomethacin in rabbit plasma by HPLC-MS/MS. Protein precipitation and extraction with acetonitrile were used for analyte isolation from plasma according to the QuEChERS principle. The target quantitative ion pairs m/z were respectively 496.4 / 358.0 for indomenthyl, 358.0 / 139.5 for indomethacin, and 340.1 / 202.1 for the IS. Conclusion: The calibration curve was linear over the range 0.1-1000 ng mL1 . The technique was applied to the pharmacokinetic study at a dose of 25 mg kg1 to rabbits
Язык:английский
Опубликовано: 2021
Предметы:
электронный ресурс
труды учёных ТПУ
Online-ссылка:https://doi.org/10.1039/D1AY01220G
Формат: Электронный ресурс Статья
Запись в KOHA:https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=657605
Описание
Примечания:Title screen
Background: Indomenthyl is an innovative anti-inflammatory drug with a high analgesic activity. Indomenthyl releases indomethacin under the influence of neutrophil esterases in the inflammation focus. Methodology/results: This research is aimed at developing a highly sensitive method for the quantitative determination of indomenthyl and its active metabolite indomethacin in rabbit plasma by HPLC-MS/MS. Protein precipitation and extraction with acetonitrile were used for analyte isolation from plasma according to the QuEChERS principle. The target quantitative ion pairs m/z were respectively 496.4 / 358.0 for indomenthyl, 358.0 / 139.5 for indomethacin, and 340.1 / 202.1 for the IS. Conclusion: The calibration curve was linear over the range 0.1-1000 ng mL1 . The technique was applied to the pharmacokinetic study at a dose of 25 mg kg1 to rabbits
DOI:10.1039/D1AY01220G

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