Quantitative determination of new anti-inflammatory drug indomenthyl and its metabolite in rabbit plasma by HPLC-MS/MS
| Parent link: | Analytical Methods Vol. 13, iss. 41.— 2021.— [9 p.] |
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| Corporate Author: | |
| Other Authors: | , , , , , , , , |
| Summary: | Title screen Background: Indomenthyl is an innovative anti-inflammatory drug with a high analgesic activity. Indomenthyl releases indomethacin under the influence of neutrophil esterases in the inflammation focus. Methodology/results: This research is aimed at developing a highly sensitive method for the quantitative determination of indomenthyl and its active metabolite indomethacin in rabbit plasma by HPLC-MS/MS. Protein precipitation and extraction with acetonitrile were used for analyte isolation from plasma according to the QuEChERS principle. The target quantitative ion pairs m/z were respectively 496.4 / 358.0 for indomenthyl, 358.0 / 139.5 for indomethacin, and 340.1 / 202.1 for the IS. Conclusion: The calibration curve was linear over the range 0.1-1000 ng mL1 . The technique was applied to the pharmacokinetic study at a dose of 25 mg kg1 to rabbits |
| Published: |
2021
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| Subjects: | |
| Online Access: | https://doi.org/10.1039/D1AY01220G |
| Format: | Electronic Book Chapter |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=657605 |