Определение чувствительности спорообразующих бактерий к антибиотику пиоцианину
| Parent link: | Перспективы развития фундаментальных наук=Prospects of Fundamental Sciences Development: сборник научных трудов XVI Международной конференции студентов, аспирантов и молодых ученых, г. Томск, 23-26 апреля 2019 г./ Национальный исследовательский Томский политехнический университет (ТПУ) ; под ред. И. А. Курзиной, Г. А. Вороновой.— , 2019 Т. 4 : Биология и фундаментальная медицина.— 2019.— [С. 134-136] |
|---|---|
| Hovedforfatter: | |
| Institution som forfatter: | |
| Andre forfattere: | |
| Summary: | Заглавие с экрана Isolation of new antibiotics from substances that weren't previously used as antimicrobial drugs may be effective against resistant bacteria. Pseudomonas aeruginosa is a producer one of these substances. Pyocyanin is the blue-green pigment of the phenazine series and the secondary metabolite. In the present study, a five-day cultivation of P. aeruginosa, extraction and analysis of pyocyanine was carried out. Confirmation of the pyocyanin was done through UV-visible spectrophotometric analysis. In UV-Vis spectrophotometric analysis, a maximum absorption was observed at 690nm. Pyocyanin was used to determine the minimum inhibitory concentration against spore-forming bacteria Bacillus cereus and Bacillus subtilis. The serial dilution method showed that for Bacillus cereus the minimum inhibitory concentration of pyocyanin is 29 [mu]g/ml, and for Bacillus subtilis 17,8 [mu]g/ml. It was detected, that pyocyanin can suppress the growth of spore-forming bacteria. |
| Sprog: | russisk |
| Udgivet: |
2019
|
| Fag: | |
| Online adgang: | http://earchive.tpu.ru/handle/11683/55934 |
| Format: | Electronisk Book Chapter |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=629770 |