Влияние нокаута генов ADAMTS1, RBFOX2 и THBS1 на транскрипционную регуляцию и клональную выживаемость клеток в модельной системе in vitro
| Parent link: | Перспективы развития фундаментальных наук=Prospects of Fundamental Sciences Development: сборник научных трудов XV Международной конференции студентов, аспирантов и молодых ученых, г. Томск, 24-27 апреля 2018 г./ Национальный исследовательский Томский политехнический университет (ТПУ) ; под ред. И. А. Курзиной, Г. А. Вороновой.— , 2018 Т. 4 : Биология и фундаментальная медицина.— 2018.— [С. 108-109] |
|---|---|
| Main Author: | |
| Corporate Authors: | , |
| Other Authors: | , |
| Summary: | Заглавие с экрана In this study, we evaluated the expression level and clonogenic survival of cell lines with a mutation of the ADAMTS1, RBFOX2, and THBS1 genes generated by CRISPR/Cas9 technology. The gene expression profile was analyzed by expression microarray. It was found that knockout of all analyzed genes leads to a decrease in their own expression. In addition, the knockout of these genes leads to significant changes in the expression of other genes in the cell lines. Clonogenic survival of the lines was decreased in the cell lines with knockout of ADAMTS1 and RBFOX2 genes after exposure to 2 Gy of [gamma]-rays. |
| Published: |
2018
|
| Subjects: | |
| Online Access: | http://earchive.tpu.ru/handle/11683/50901 |
| Format: | Electronic Book Chapter |
| KOHA link: | https://koha.lib.tpu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=627665 |