Bilaketaren emaitzak - "WWE"

ADP-ribosylation and NAD+ Utilizing Enzymes Methods and Protocols /

Aurkibidea: “...Vitamin B3 in Health and Disease: Toward the Second Century of Discovery -- Monitoring Poly(ADP-Ribosyl)ation in Response to DNA Damage in Live Cells Using Fluorescently-Tagged Macrodomains -- In Vitro Techniques for ADP-Ribosylated Substrate Identification -- Assessment of Intracellular Auto-Modification Levels of ARTD10 using Mono-ADP-Ribose-Specific Macrodomains 2 and 3 of Murine Artd8 -- Biochemical and Biophysical Assays of PAR-WWE Domain Interactions and Production of iso-ADPr for PAR Binding Analysis -- Assays for NAD-Dependent Reactions and NAD Metabolites -- Generating Protein-Linked and Protein-Free Mono-, Oligo-, and Poly(ADP-Ribose) In Vitro -- Methods to Study TCDD-Inducible Poly-ADP-Ribose Polymerase (TIPARP) Mono-ADP-Ribosyltransferase Activity -- Dictyostelium as a Model to Assess Site-Specific ADP-Ribosylation Events -- Mono-ADP-Ribosylation Catalyzed by Arginine-Specific ADP-Ribosyltransferases -- Monitoring Expression andEnzyme Activity of Ecto-ARTCs -- ADP-Ribosyl-Acceptor Hydrolase Activities Catalyzed by the ARH Family of Proteins -- Mono-ADP-Ribosylhydrolase Assays -- Hydrolysis of ADP-Ribosylation by Macrodomains -- HPLC-Based Enzyme Assays for Sirtuins -- Small Molecule Screening Assay for Mono-ADP-Ribosyltransferases -- A Simple, Sensitive, and Generalizable Plate Assay for Screening PARP Inhibitors -- Non-Localized Searching of HCD Data for Fast and Sensitive Identification of ADP-Ribosylated Peptides -- Quantitative Determination of MAR Hydrolase Residue Specificity In Vitro by Tandem Mass Spectrometry -- Detection of ADP-Ribosylating Bacterial Toxins -- Preparation of Recombinant Alphaviruses for Functional Studies of ADP-Ribosylation -- Monitoring the Sensitivity of T Cell Populations towards NAD+ Released during Cell Preparation -- Identifying Target RNAs of PARPs -- ADPr-Peptide Synthesis -- Identifying Genomic Sites of ADP-Ribosylation Mediated by Specific Nuclear PARP Enzymes Using Click-ChIP -- Methods for Using a Genetically-Encoded Fluorescent Biosensor to Monitor Nuclear NAD+....”
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