Search Results - "HIV"

HIV Reservoirs Methods and Protocols /

Table of Contents: “…Myeloid Cell Lines as Surrogate Models Of Reversible Proviral Latency -- An In Vitro System to Model the Establishment and Reactivation of HIV-1 Latencyin primary human CD4+ T cells -- The cultured TCM model of HIV latency -- A Reliable Primary Cell Model for HIV latency: The QUECEL (Quiescent Effector Cell Latency) Method -- TGF-b signaling supports HIV latency in a Memory CD4+ T cell based in vitro model.p Flow Cytometry Sorting of Memory CCR6+CD4+ T-Cells for HIV Reservoirs Quantification -- Human monocyte-derived macrophages (MDM) - Model 1 (GM-CSF) -- Human monocyte-derived macrophages (MDM) - Model 2 -- Modeling HIV latency in astrocytes with the human neural progenitor cell line HNSC.100 -- Hematopoietic stem and progenitor cells (HSPCs) -- HIV reservoirs: Modeling, Quantification and Approaches to a Cure -- More than a gender issue: Testis as a distinctive HIV reservoir and its implication for viral eradication -- Experimental Models to Study HIV Latency Reversal from Male Genital Myeloid Cells -- Decidua basalis: An ex vivo Model to Study HIV-1 Infection during Pregnancy and Beyond -- HIV Reservoirs: Modeling, Quantification, and Approaches to a Cure -- Human Hematopoietic Stem Cell (HSC)-Engrafted NSG Mice for HIV Latency Research -- Inducing long-term HIV-1 latency in the TKO-BLT mouse model -- In situ multiplexing to identify, quantify, and phenotype the HIV-1/SIV reservoir within lymphoid tissue -- Single-cell multiparametric analysis of rare HIV-infected cells identified by duplexed RNAflow-FISH -- Ex vivo differentiation of resting CD4+ T lymphocytes enhances detection of replication competent HIV-1 in viral outgrowth assays -- Quantitative Viral Outgrowth Assay to Measure the functional SIV Reservoir in Myeloid Cells -- Near-full-length single-genome HIV-1 DNA sequencing -- TILDA: Tat/rev Induced Limiting Dilution Assay -- Latency Reversal and Clearance of Persistent HIV Infection -- Cure and long-term remission strategies -- Pathways Toward a Functional HIV-1 Cure: Balancing Promise and Perils for CRISPR Therapy. .…”
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HIV Protocols

Table of Contents: “…Infectious Virus Tracking by Fluorescent Live Cell Imaging in Primary Cells -- Imaging HIV-1 Nuclear Import, Uncoating, and Proviral Transcription -- RNA-FISH for HIV Transcription/Localization Analysis -- Single-Cell and Single-Molecule RNA-FISH Combined with Immunofluorescence and High-Speed and High-Resolution Scanning Analysis to Visualize Reactivation of Latent HIV-1 -- Visualizing HIV-1 Assembly at the T Cell Plasma Membrane using Single Molecule Localization: TIRF Microscopy -- Single-Virion Analysis: A Method to Visualize HIV-1 Particle Content Using Fluorescence Microscopy -- Correlative Imaging to Detect Rare HIV Reservoirs and Associated Damage in Tissues -- Characterization of Nuclear HIV-Induced Membraneless Organelles through Fluorescence Microscopy -- Detection of CPSF6 in Biomolecular Condensates as a Reporter of Nuclear Import -- Monitoring HIV-1 Nuclear Import Kinetics Using a Chemically Induced Nuclear Pore Blockade Assay -- Biochemical Detection of Capsid in the Nucleus During HIV-1 Infection -- Chromatin Immunoprecipitation of Retroviral Genomes with Antibodies Recognizing Modified Histones and Specific Viral Proteins -- Measurement of HIV Rev-Rev Response Element Functional Activity -- Method for the Enrichment of N6-Methyladenosine-Modified Cellular and HIV-1 RNA -- Direct Analysis of HIV mRNA m6A Methylation by Nanopore Sequencing -- Identifying Individual Pseudouridine (Ψ) Sites Across Transcripts from HIV-1 Infected Cells -- Proximity Ligation Assay to Detect the Proximity between Host Proteins and Viral Proteins of HIV-1 -- Modeling HIV-1 Infection in CNS via Infected Monocytes using Immunocompetent Brain Organoids -- An In Vitro Model of Blood Brain Barrier for Studies on HIV Neuroinflammation and CNS Antibody Penetration -- Generation of Anti-HIV CAR-T Cells for Preclinical Research -- Highly Sensitive Analysis of Cervical Mucosal HIV-1 Infection using Reporter Viruses Expressing Secreted Nanoluciferase -- Multiparameter Flow Cytometry Monitoring of T Cell Responses -- An Ultrasensitive p24 Assay to Measure HIV-1 in Diverse Biological Matrixes.…”
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HIV Protocols

Table of Contents: “…Quantifying CD4/CCR5 Usage Efficiency of HIV-1 ENV Using the Affinofile System -- Measuring T Cell-to-T Cell HIV-1 Transfer, Viral Fusion, and Infection Using Flow Cytometry -- HIV-1 Capsid Stabilization Assay -- Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Viral Core Integrity -- HIV-1 Reverse Transcriptase Based Assay to Determine Cellular dNTP Concentrations -- Rapid Determination of HIV-1 Mutant Frequencies and Mutation Spectra Using an mCherry/EGFP Dual Reporter Viral Vector -- Novel Biochemical Tools for Probing HIV RNA Structure -- Analysis of HIV-1 Gag-RNA Interactions in Cells and Virions by CLIP-seq -- Isolation of Cognate Cellular and Viral Ribonucleoprotein Complexes of HIV-1 RNA Applicable to Proteomic Discovery and Molecular Investigations -- Methods for the Analyses of Inhibitor-Induced Aberrant Multimerization of HIV-1 Integrase -- Quantification of HIV-1 Gag Localization Within Virus Producer Cells -- Methods to Study Determinants for Membrane Targeting of HIV-1 Gag In Vitro -- Visualizing the Behavior of HIV-Infected T Cells In Vivo Using Multiphoton Intravital Microscopy -- Modeling HIV-1 Mucosal Transmission and Prevention in Humanized Mice -- High Throughput Humanized Mouse Models for Evaluation of HIV-1 Therapeutics and Pathogenesis -- Measuring the Frequency of Latent HIV-1 in Resting CD4+ T Cells Using a Limiting Dilution Co-Culture Assay -- LGIT In Vitro Latency Model in Primary and T Cell Lines to Test HIV-1 Reactivation Compounds -- Improved Methods to Detect Low Levels of HIV Using Antibody-Based Technologies -- Analysis of ABCA1 and Cholesterol Efflux in HIV-Infected Cells -- The Proteomic Characterization of Plasma or Serum from HIV-Infected Patients -- Proteomic Characterization of Exosomes from HIV-1-Infected Cells -- Detecting HIV-1 Tat in Cell Culture Supernatants by ELISA or Western Blot -- Protocol for Detection of HIV-Tat Protein in Cerebrospinal Fluid by a Sandwich Enzyme Linked Immunosorbent Assay -- Measuring the Uptake and Transactivation Function of HIV-1 Tat Protein in a Trans-Cellular Co-Cultivation Set Up -- Evaluating the Role of Viral Proteins in HIV-Mediated Neurotoxicity Using Primary Human Neuronal Cultures.…”
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HIV Protocols

Table of Contents: “…Virology -- Isolation and Expansion of HIV from Cells and Body Fluids by Coculture -- Quantitative HIV Culture -- Quantitation of Cell-Free HIV by Reverse Transcriptase Activity -- Determination of Syncytium-Inducing Phenotype of Primary HIV-1 Isolates Using MT-2 cells -- Determination of HIV-1 Chemokine Coreceptor Tropism Using Transduced Human Osteosarcoma (HOS) Cells -- Use of Luciferase Reporter Viruses for Studying HIV Entry -- A Cell-Cell Fusion Assay to Monitor HIV-1 Env Interactions with Chemokine Receptors -- Methods of Culturing HIV-1 from Semen -- Molecular Biology -- Detection of HIV-1 Nucleic Acids by Southern Blotting -- Detection of HIV-1 Nucleic Acids by Northern Blotting -- Rapid Identification of Cloned HIV-1 Fragments -- PCR Amplification and Cloning of Virtually Full-Length HIV-1 Provirus -- Quantitation of HIV-1gag DNA and RNA From Single Frozen Cell Pellets -- Quantitative PCR for HIV-1 Proviral DNA -- Diagnosis and Direct Automated Sequencing of HIV-1 From Dried Blood Spots (DBS) Collected on Filter Paper -- Quantification of HIV-1 RNA in Dried Plasma Spots (DPS) -- Quantitation of HIV-1 RNA in Genital Secretions -- Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase Chain Reaction -- Determination of the Promoter Activity of HIV-1 Using the Chloramphenicol Acetyltransferase Reporter Gene Assay -- In Vitro Techniques for Studies of HIV-1 Promoter Activity -- Detection of Polymorphisms in the HIV-1 Coreceptor CCR5 Using Single-Strand Conformation Polymorphism -- Sequence-Specific Priming as a Rapid Screen for Known Mutations -- Quantitation of HIV-1 Entry Cofactor Expression -- Retrovirus-Mediated Gene Transduction of SupT1 Cells -- Cloning of HIV Single-Strand DNA Binding Protein from Human Lymphocyte Lambda gt11 Expression Library with [32P]-Oligomers.…”
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HIV Protocols Second Edition /

Table of Contents: “…Preparation of Virus Particles and their Analysis -- Methods for Viral RNA Isolation and PCR Amplification for Sequencing of Near Full-Length HIV-1 Genomes -- Purification of HIV-1 Virions by Subtilisin Digestion or CD45 Immunoaffinity Depletion for Biochemical Studies -- Calculating HIV-1 Infectious Titre Using a Virtual TCID50 Method -- Methods To Study Hiv-1 Replication -- Cell-Free Assays for HIV-1 Uncoating -- Real-Time PCR Analysis of HIV-1 Replication Post-entry Events -- Analysis of 2-LTR Circle Junctions of Viral DNA in Infected Cells -- HIV-1 Recombination: An Experimental Assay and a Phylogenetic Approach -- Methods of Preparation and Analysis of Intracellular Reverse Transcription Complexes -- Analysis of Viral and Cellular Proteins in HIV-1 Reverse Transcription Complexes by Co-immunoprecipitation -- Isolation and Analysis of HIV-1 Preintegration Complexes -- Bisarsenical Labeling of HIV-1 for Real-Time Fluorescence Microscopy -- Methods for the Study of HIV-1 Assembly -- Assembly of Immature HIV-1 Capsids Using a Cell-Free System -- Preparation of Recombinant HIV-1 Gag Protein and Assembly of Virus-Like Particles In Vitro -- Methods for the Analysis of HIV-1 Nucleocapsid Protein Interactions with Oligonucleotides -- Methods for Analysis of Incorporation and Annealing Methods for Analysis of tRNALys in HIV-1 -- Specialized Approaches To Study Hiv-1 Biology and Pathogenesis -- Somatic Cell Genetic Analyses to Identify HIV-1 Host Restriction Factors -- Rapid, Controlled and Intensive Lentiviral Vector-Based RNAi -- Reverse Two-Hybrid Screening to Analyze Protein–Protein Interaction of HIV-1 Viral and Cellular Proteins -- Methods to Study Monocyte Migration Induced by HIV-Infected Cells -- Novel Mouse Models for Understanding HIV-1 Pathogenesis -- Immunological Studies Of Hiv.…”
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The Eroticizing of HIV Viral Fantasies / by García-Iglesias, Jaime

Table of Contents: “…Medicated Fantasies: After HIV diagnosis -- 7. Conclusion: Thinking Virus, Thinking Fantasy.…”
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HIV Survivors in Sydney Memories of the Epidemic / by Ware, Cheryl

Table of Contents: “…The Gay Capital of the Southern Hemisphere -- 3. The Face of HIV -- 4. 'The Disease of a Thousand Rehearsals' -- 5. …”
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Trans Women and HIV Social Psychological Perspectives / by Jaspal, Rusi

Table of Contents: “…Understanding Trans Women and HIV -- 2.The Construction of Trans Identity -- 3.HIV Stressors and Risk Factors -- Part 2:Trans Women Living with HIV in the UK -- 4.Researching HIV and Trans Women -- 5.Multi-Layered Stigma and Identity Threat -- 6.Self-Isolation and HIV Disclosure -- 7: Sex Work and HIV -- Part 3:Conclusion -- 8.Supporting Trans Women Living with HIV.…”
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HIV/AIDS in Bangladesh Stigmatized People, Policy and Place / by Paul, Alak

Table of Contents: “…Chapter 1-HIV/AIDS in Bangladesh: Present Research -- Chapter 2-'Lifeworlds’ of Marginalized People in HIV Discourse -- Chapter 3-Stigmatized People and Societal Prejudice -- Chapter 4-HIV Risk Behaviour, Consciousness and ‘Risk Coping’ -- Chapter 5-Place, Mobility and Channelling of HIV Risk -- Chapter 6-Addressing HIV Issues: National Policy to Local Practice -- Chapter 7-Summary and Conclusion.…”
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HIV/AIDS and Adolescents South Pacific and Caribbean / by Misir, Prem

Table of Contents: “…Chapter 1 Introduction -- Chapter 2 Systematic Review of the Literature -- Chapter 3 Methodology -- Chapter 4 HIV/AIDS Knowledge -- Chapter 5 HIV/AIDS Knowledge and Socio-demographics -- Chapter 6 Predictors of Knowledge & Stigma-related Attitudes/Perceptions -- Chapter 7 HIV/AIDS Stigma and Socio-demographics -- Chapter 8 Discussion and Implications.…”
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HIV and Gay Men Clinical, Social and Psychological Aspects / by Jaspal, Rusi, Bayley, Jake

Table of Contents: “…Chapter 1: Understanding the Clinical and Social Psychological Aspects of HIV -- Chapter 2: HIV: Its History, Science & Epidemiology -- Chapter 3: Sexuality & HIV Risk in Gay Men -- Chapter 4: HIV Prevention -- Chapter 5: HIV Diagnosis, Management & Prognosis -- Chapter 6: HIV & Mental Health -- Chapter 7: Intersecting Identities -- Chapter 8: Looking to the Future – Eradication by 2030?.…”
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Gender and HIV in South Africa Advancing Women’s Health and Capabilities / by Sprague, Courtenay

Table of Contents: “…Introduction -- Part I. HIV, Gender and Health for Black South African Women -- 1. …”
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Working with Excluded Populations in HIV Hard to Reach or Out of Sight? / by Logie, Carmen

“…Social Aspects of HIV,…”
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Aging with HIV in Sub-Saharan Africa Health and Psychosocial Perspectives /

Table of Contents: “…Chapter 1. The Epidemiology of HIV in the Older African Population -- Chapter 2. …”
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HIV/AIDS in Memory, Culture and Society

Table of Contents: “…Viral Echoes: Revisiting the Cultural Memory of HIV/AIDS - Alicia Castillo Villanueva & Angelos Bollas -- 2. …”
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A Clinical Guide to Pediatric HIV Bridging the Gaps Between Research and Practice /

Table of Contents: “…Epidemiology of Pediatric HIV Infection -- Chapter 2. Medical Implications of HIV among Children and Adolescents -- Chapter 3. …”
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Politics in the Making of HIV/AIDS in South Africa by Pienaar, K.

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Remaking HIV Prevention in the 21st Century The Promise of TasP, U=U and PrEP /

Table of Contents: “…Making the ideal real: Biomedical HIV prevention as social public health (Mark Davis) -- Chapter 4. …”
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Children and Young People Living with HIV/AIDS A Cross-Cultural Perspective /

Table of Contents: “…Children, young people and HIV/AIDS: An Introduction; Pranee Liamputtong -- Part I. …”
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Understanding the HIV/AIDS Epidemic in the United States The Role of Syndemics in the Production of Health Disparities /

Table of Contents: “…Chapter 1. Understanding the HIV/AIDS Epidemic in the United States: The Role of Syndemics in Shaping the Public’s Health; Eric R. …”
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