Calcium Signaling Protocols

Dades bibliogràfiques
Autor corporatiu: SpringerLink (Online service)
Altres autors: Lambert, David G. (Editor), Rainbow, Richard D. (Editor)
Sumari:XI, 360 p. 51 illus., 17 illus. in color.
text
Idioma:anglès
Publicat: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Edició:3rd ed. 2013.
Col·lecció:Methods in Molecular Biology, 937
Matèries:
Accés en línia:https://doi.org/10.1007/978-1-62703-086-1
Format: Electrònic Llibre
Taula de continguts:
  • Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations
  • Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2
  • Confocal Microscopy: Theory and Applications for Cellular Signaling
  • Ratiometric Ca2+ Measurements Using the FlexStation® Scanning Fluorometer
  • Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader (FLIPR®)
  • Ratiometric [Ca2+]i Measurements in Adherent Cell-lines Using the NOVOstar Microplate Reader
  • Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents in Single Cells: Heterologous Expression Systems and Neurones
  • Combined Calcium Fluorescence Recording with Ionic Currents in Contractile Cells
  • Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol Labeling Protocols in Permeabilized Cells
  • Single Cell Imaging Techniques for the Real-Time Detection of IP3 in Live Cells
  • Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay
  • Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry
  • Calcium Measurements from Whole Heart Using Rhod-2.- Measurement of Changes in Endothelial and Smooth Muscle Ca2+ in Pressurized Arteries
  • Single Cell and Subcellular Measurements of Intracellular Ca2+ Concentration
  • Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations
  • Measurements of Ca2+ Concentration with Recombinant Targeted Luminescent Probes
  • Chimeric G-Proteins in Fluorimetric Calcium Assays: Experience with Opioid Receptors
  • Compartmentalizing Genetically-Encoded Calcium Sensors
  • Measurement of Cytosolic-Free Ca2+ in Plant Tissue
  • Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1).