Mitochondrial Bioenergetics Methods and Protocols /
| Korporativní autor: | |
|---|---|
| Další autoři: | , |
| Shrnutí: | XI, 333 p. 83 illus., 25 illus. in color. text |
| Jazyk: | angličtina |
| Vydáno: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2012.
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| Vydání: | 1st ed. 2012. |
| Edice: | Methods in Molecular Biology,
810 |
| Témata: | |
| On-line přístup: | https://doi.org/10.1007/978-1-61779-382-0 |
| Médium: | Elektronický zdroj Kniha |
Obsah:
- Overview of Mitochondrial Bioenergetics.- Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells.- High-Resolution Respirometry. OXPHOS Protocols for Human Cells and Permeabilized Fibres from Small Biopsies of Human Muscle
- High Throughput Analysis of Mitochondrial Oxygen Consumption. – Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices. - Mitochondrial Membrane Potential (DY) Fluctuations Associated with the Metabolic States of Mitochondria. - Safranine as a Fluorescent Probe for the Evaluation of Mitochondrial Membrane Potential in Isolated Organelles and Permeabilized Cells
- Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells.-Phenomenological Kinetic and Control Analysis of Oxidative Phosphorylation in Isolated Mitochondria.-Expression of Uncoupling Proteins in a Mammalian Cell Culture System (HEK293) and Assessment of their Protein Function.-Measurement of Proton Leak and Electron Leak in Isolated Mitochondria.-Relation between Mitochondrial Membrane Potential and ROS Formation
- Use of a Calcium-Sensitive Electrode for Studies on Mitochondrial Calcium Transport .-Imaging Mitochondrial Calcium Signalling with Fluorescent Probes and Single or Two Photon Confocal Microscopy
- Mitochondrial Permeability Transition Pore and Calcium Handling.- Imaging of Mitochondrial pH Using SNARF-1
- Redox Equivalents and Mitochondrial Bioenergetics.-NMR Methodologies for Studying Mitochondrial Bioenergetics.-Computational Modeling of Mitochondrial Function.