Plant Kinases Methods and Protocols /

Podrobná bibliografie
Korporativní autor: SpringerLink (Online service)
Další autoři: Dissmeyer, Nico (Editor), Schnittger, Arp (Editor)
Shrnutí:X, 285 p. 39 illus.
text
Jazyk:angličtina
Vydáno: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Vydání:1st ed. 2011.
Edice:Methods in Molecular Biology, 779
Témata:
On-line přístup:https://doi.org/10.1007/978-1-61779-264-9
Médium: Elektronický zdroj Kniha
Obsah:
  • Guide to the Book Plant Kinases
  • The Age of Protein Kinases
  • Expression and Purification of Active Protein Kinases from Wheat Germ Extracts
  • Measurement of Plant Cyclin-Dependent Kinase Activity Using Immunoprecipitation-Coupled and Affinity Purification-Based Kinase Assays and the Baculovirus Expression System
  • Mitogen-Activated Protein Kinase Activity and Reporter Gene Assays in Plants
  • Use of Phospho-site Substitutions to Analyze the Biological Relevance of Phosphorylation Events in Regulatory Networks
  • Bacterial Assay to Study Plant Sensor Histidine Kinases.-  Substrate Analysis of Arabidopsis PP2C-type Protein Phosphatase
  • Modulating and Monitoring MAPK Activity During Programmed Cell Death in Pollen
  • Sensitizing Plant Protein Kinases to Specific Inhibition by ATP-Competitive Molecules
  • Fluorescence Fluctuation Analysis of Receptor Kinase Dimerization
  • Quantifying Degradation Rates of Trans-Membrane Receptor Kinases
  • Fluorescence Correlation Spectroscopy and Fluorescence Recovery after Photobleaching to Study Receptor Kinase Mobility in planta
  • Bimolecular-Fluorescence Complementation Assay to Monitor Kinase-Substrate Interactions in vivo
  • Chemical Genetic Analysis of Protein Kinase Function in Plants
  • Modified Metal Oxide Affinity Enrichment Combined with 2D-PAGE and Analysis of Phosphoproteomes
  • Phosphoproteomics Using iTRAQ.