Transgenic Mouse Methods and Protocols
| Ente Autore: | |
|---|---|
| Altri autori: | , |
| Riassunto: | XI, 352 p. text |
| Lingua: | inglese |
| Pubblicazione: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2011.
|
| Edizione: | 2nd ed. 2011. |
| Serie: | Methods in Molecular Biology,
693 |
| Soggetti: | |
| Accesso online: | https://doi.org/10.1007/978-1-60761-974-1 |
| Natura: | Elettronico Libro |
Sommario:
- Introduction: Strategies for developing genetically modified mice
- Genetic Modification of the Mouse; General Technology: Pronuclear and Blastocyst Injection
- Generation of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos
- Cryopreservation of mouse spermatozoa and In vitro fertilization
- Autopsy and histological analysis of the transgenic mouse
- Transgene design
- Inducible Transgenic Mouse Models
- Lentiviral transgenesis
- Transgenesis in Mouse Embryonic Stem Cells
- Engineering BAC Reporter Gene Constructs for Mouse Transgenesis
- Targeting Vector Construction Through Recombineering
- Generating Conditional Knockout Mice
- Hypomorphic mice
- MICER Targeting Vectors for Manipulating the Mouse Genome
- Knockin approaches
- Generation of a series of knockin alleles using RMCE in ES cells
- Selection of targeted mutants from a library of randomly mutagenized ES cells
- Generation of genetically modified rodents using random ENU mutagenesis
- Bone marrow transplantations to study gene function in hematopoietic cells
- Adenovirus Mediated Gene Transfer.