Plant Developmental Biology Methods and Protocols /

书目详细资料
企业作者: SpringerLink (Online service)
其他作者: Hennig, Lars (Editor), Köhler, Claudia (Editor)
总结:XI, 448 p.
text
语言:英语
出版: Totowa, NJ : Humana Press : Imprint: Humana, 2010.
版:1st ed. 2010.
丛编:Methods in Molecular Biology, 655
主题:
在线阅读:https://doi.org/10.1007/978-1-60761-765-5
格式: 电子 图书
书本目录:
  • Growth Protocols for Model Plants in Developmental Biology
  • Grafting as a Research Tool
  • Virus-Induced Gene Silencing as a Reverse Genetics Tool to Study Gene Function
  • The CRE/lox System as a Tool for Developmental Studies at the Cell and Tissue Level
  • Inducible Gene Expression Systems for Plants
  • Trichome Development in Arabidopsis
  • Phenotyping the Development of Leaf Area in Arabidopsis thaliana
  • Analyzing Shoot Apical Meristem Development
  • Analyzing Floral Meristem Development
  • Female Gametophytic Mutants: Diagnosis and Characterization
  • Pollen Tube Development
  • Analysis of Root Meristem Size Development
  • Phenotypic Characterization of Photomorphogenic Responses During Plant Development
  • Kinematic Analysis of Cell Division and Expansion
  • Flowering Time Control
  • mRNA Detection by Whole Mount In Situ Hybridization (WISH) or Sectioned Tissue In Situ Hybridization (SISH) in Arabidopsis
  • Immunolocalization of Proteins in Plants
  • Detection of Small Non-coding RNAs
  • Quantitative Real Time PCR in Plant Developmental Biology
  • Luciferase and Green Fluorescent Protein Reporter Genes as Tools to Determine Protein Abundance and Intracellular Dynamics
  • Fluorescence-Activated Cell Sorting in Plant Developmental Biology
  • Laser Microdissection of Paraffin-Embedded Plant Tissues for Transcript Profiling
  • Utilizing Bimolecular Fluorescence Complementation (BiFC) to Assay Protein–Protein Interaction in Plants
  • The Split Luciferase Complementation Assay
  • Co-immunoprecipitation and Protein Blots
  • Probing Protein–Protein Interactions with FRET–FLIM
  • Plant Chromatin Immunoprecipitation
  • Immunocytological Analysis of Chromatin in Isolated Nuclei
  • Bisulphite Sequencing of Plant Genomic DNA.