In Vitro Mutagenesis Protocols Third Edition /
| Autor Corporativo: | |
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| Outros Autores: | |
| Resumo: | XIV, 442 p. text |
| Idioma: | inglês |
| Publicado em: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2010.
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| Edição: | 3rd ed. 2010. |
| Colecção: | Methods in Molecular Biology,
634 |
| Assuntos: | |
| Acesso em linha: | https://doi.org/10.1007/978-1-60761-652-8 |
| Formato: | Recurso Electrónico Livro |
Sumário:
- Mutagenesis in Various Microbial Backgrounds
- Mutagenesis Protocols in Saccharomyces cerevisiae by In Vivo Overlap Extension
- In Vitro Mutagenesis of Brucella Species
- Random Mutagenesis Strategies for Campylobacter and Helicobacter Species
- Mutagenesis of the Repeat Regions of Herpesviruses Cloned as Bacterial Artificial Chromosomes
- An Efficient Protocol for VZV BAC-Based Mutagenesis
- A Method for Rapid Genetic Integration into Plasmodium falciparum Utilizing Mycobacteriophage Bxb1 Integrase
- PCR Mutagenesis
- Random Mutagenesis by Error-Prone PCR
- A Rapid and Versatile PCR-Based Site-Directed Mutagenesis Protocol for Generation of Mutations Along the Entire Length of a Cloned cDNA
- Rapid Sequence Scanning Mutagenesis Using In Silico Oligo Design and the Megaprimer PCR of Whole Plasmid Method (MegaWHOP)
- Insertion and Deletion Mutagenesis by Overlap Extension PCR
- Targeted Amplification of Mutant Strands for Efficient Site-Directed Mutagenesis and Mutant Screening
- A Modified Inverse PCR Procedure for Insertion, Deletion, or Replacement of a DNA Fragment in a Target Sequence and Its Application in the Ligand Interaction Scan Method for Generation of Ligand-Regulated Proteins
- Amplification of Orthologous Genes Using Degenerate Primers
- Reviews
- Computational Evaluation of Protein Stability Change upon Mutations
- Approaches for Using Animal Models to Identify Loci That Genetically Interact with Human Disease-Causing Point Mutations
- Protein Evolution Mutagenesis
- Using Peptide Loop Insertion Mutagenesis for the Evolution of Proteins
- Massive Mutagenesis®: High-Throughput Combinatorial Site-Directed Mutagenesis
- Directed In Vitro Evolution of Reporter Genes Based on Semi-Rational Design and High-Throughput Screening
- Ribosome Display for RapidProtein Evolution by Consecutive Rounds of Mutation and Selection
- Protein Structure and Function Mutagenesis
- Fine-Tuning Enzyme Activity Through Saturation Mutagenesis
- Characterization of Structural Determinants of Type 1 Corticotropin Releasing Hormone (CRH) Receptor Signalling Properties
- Site-Directed Mutagenesis for Improving Biophysical Properties of VH Domains
- Phenotype Based Functional Gene Screening Using Retrovirus-Mediated Gene Trapping in Quasi-Haploid RAW 264.7 Cells
- Site-Directed Disulfide Cross-Linking to Probe Conformational Changes of a Transporter During Its Functional Cycle: Escherichia coli AcrB Multidrug Exporter as an Example
- Site-Specific Incorporation of Extra Components into RNA by Transcription Using Unnatural Base Pair Systems
- Random Mutagenesis
- Mutagen™: A Random Mutagenesis Method Providing a Complementary Diversity Generated by Human Error-Prone DNA Polymerases
- Random-Scanning Mutagenesis
- Easy Two-Step Method for Randomizing and Cloning Gene Fragments
- Mutator Bacterial Strain Mutagenesis
- Random Mutagenesis Using a Mutator Strain
- En Passant Mutagenesis: A Two Step Markerless Red Recombination System.