Avidin-Biotin Interactions Methods and Applications /

Bibliografiske detaljer
Institution som forfatter: SpringerLink (Online service)
Andre forfattere: McMahon, Robert J. (Editor)
Summary:XIV, 232 p. 38 illus.
text
Sprog:engelsk
Udgivet: Totowa, NJ : Humana Press : Imprint: Humana, 2008.
Udgivelse:1st ed. 2008.
Serier:Methods in Molecular Biology, 418
Fag:
Online adgang:https://doi.org/10.1007/978-1-59745-579-4
Format: Electronisk Bog
Indholdsfortegnelse:
  • Preparation of Avidin Conjugates
  • Coupling of Antibodies With Biotin
  • Optimization of Detection and Quantification of Proteins on Membranes in Very High and Very Low Abundance Using Avidin and Streptavidin
  • Use of the Avidin (Imino)Biotin System as a General Approach to Affinity Precipitation
  • Capturing Biotinylated Proteins and Peptides by Avidin Functional Affinity Electrophoresis
  • Functionality Screen of Streptavidin Mutants by Non-Denaturing SDS–PAGE Using Biotin-4-Fluorescein
  • Application of Biotin-4-Fluorescein in Homogeneous Fluorescence Assays for Avidin, Streptavidin, and Biotin or Biotin Derivatives
  • Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resins
  • High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed E. coli
  • Endogenous Biotin in Rat Brain
  • Pseudo-Immunolabelling With the Avidin–Biotin–Peroxidase Complex due to the Presence of Endogenous Biotin in the Retina
  • Use of Synthetic Peptides for Identifying Biotinylation Sites in Human Histones
  • Detection and Quantitation of the Activity of DNA Methyltransferases Using a Biotin/Avidin Microplate Assay
  • Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or Streptavidin
  • Mammalian Lectin as Tool in Glycochemistry and Histochemistry With Relevance for Diagnostic Procedure
  • A Multiplex Approach to Isotyping Antigen-Specific Antibodies Using Biotinylated Antigen/Streptavidin-Phycoerythrin
  • Biotin–Protein Bond: Instability and Structural Modification to Provide Stability for In Vivo Applications.