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|a 9781597453776
|9 978-1-59745-377-6
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|a 10.1385/1597453773
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|a Pyrosequencing Protocols
|h [electronic resource] /
|c edited by Sharon Marsh.
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|a 1st ed. 2007.
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2007.
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|a XII, 196 p.
|b online resource.
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|a Methods in Molecular Biology,
|x 1940-6029 ;
|v 373
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|a The History of Pyrosequencing® -- Pyrosequencing® Applications -- Web-Based Primer Design Software for Genome-Scale Genotyping by Pyrosequencing® -- Pyrosequencing® -- Universal Primer Applications for Pyrosequencing® -- Allele Quantification and DNA Pooling Methods -- Multiplex Pyrosequencing® for DNA Variation Analysis -- Analysis of Gene-Specific DNA Methylation Patterns by Pyrosequencing® Technology -- Detecting Mutations That Confer Oxazolidinone Resistance in Gram-Positive Bacteria -- Pyrosequencing®-Based Strategies for Improved Allele Typing of Human Leukocyte Antigen Loci -- Pyrosequencing® of Phage Display Libraries for the Identification of Cell-Specific Targeting Ligands -- Gene Copy Number Detection in Animal Studies -- Detection of Allelic Imbalance in Gene Expression Using Pyrosequencing® -- Pyrosequencing® Genotype Storage Techniques.
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| 520 |
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|a Pyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method. Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.
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|a Biochemistry.
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|a Medical genetics.
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|a Biochemistry.
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|a Medical Genetics.
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|a Marsh, Sharon.
|e editor.
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|a SpringerLink (Online service)
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|z 9781588296450
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|i Printed edition:
|z 9781617376931
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|a Methods in Molecular Biology,
|x 1940-6029 ;
|v 373
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|u https://doi.org/10.1385/1597453773
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