Adipose Tissue Protocols

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Yang, Kaiping (Editor)
Summary:XVI, 336 p. 61 illus., 9 illus. in color.
text
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2008.
Edition:2nd ed. 2008.
Series:Methods in Molecular Biology, 456
Subjects:
Online Access:https://doi.org/10.1007/978-1-59745-245-8
Format: Electronic Book
Table of Contents:
  • Overview of Adipose Tissue and Its Role in Obesity and Metabolic Disorders
  • Choosing an Adipose Tissue Depot for Sampling
  • Application of Imaging and Other Noninvasive Techniques in Determining Adipose Tissue Mass
  • Generation of Adipose Tissue-Specific Transgenic Mouse Models
  • Angiogenesis in Adipose Tissue
  • Adipose Organ Nerves Revealed by Immunohistochemistry#
  • Human Adipose Tissue Blood Flow and Micromanipulation of Human Subcutaneous Blood Flow
  • Studies of Thermogenesis and Mitochondrial Function in Adipose Tissues
  • Application of Lipidomics and Metabolomics to the Study of Adipose Tissue
  • Applications of Proteomics to the Study of Adipose Tissue
  • Application of DNA Microarray to the Study of Human Adipose Tissue/Cells
  • Differentiation of Adipose Stem Cells
  • Methods That Resolve Different Contributions of Clonal Expansion to Adipogenesis in 3T3-L1 and C3H10T1/2 Cells
  • Explant Cultures of White Adipose Tissue
  • Isolation and Culture of Preadipocytes from Rodent White Adipose Tissue
  • Flow Cytometry on the Stromal-Vascular Fraction of White Adipose Tissue
  • Application of Electrophoretic Mobility Shift Assay and Chromatin Immunoprecipitation in the Study of Transcription in Adipose Cells
  • Application of RNA Interference Techniques to Adipose Cell Cultures
  • RNA Isolation and Real-Time Quantitative RT-PCR
  • Study of Adipose Tissue Gene Expression by In Situ Hybridization
  • Application of Immunocytochemistry and Immunofluorescence Techniques to Adipose Tissue and Cell Cultures
  • Determination of Lipolysis in Isolated Primary Adipocytes
  • Study of Glucose Uptake in Adipose Cells
  • Measurement of Phosphoinositide 3-Kinase and Its Products to Study Adipogenic Signal Transduction.