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100301s2008 xxu| s |||| 0|eng d |
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|a 9781597452458
|9 978-1-59745-245-8
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|a 10.1007/978-1-59745-245-8
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|a 571.6
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|a Adipose Tissue Protocols
|h [electronic resource] /
|c edited by Kaiping Yang.
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|a 2nd ed. 2008.
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2008.
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|a XVI, 336 p. 61 illus., 9 illus. in color.
|b online resource.
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|a text
|b txt
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|a computer
|b c
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|a online resource
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|a text file
|b PDF
|2 rda
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|a Methods in Molecular Biology,
|x 1940-6029 ;
|v 456
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|a Overview of Adipose Tissue and Its Role in Obesity and Metabolic Disorders -- Choosing an Adipose Tissue Depot for Sampling -- Application of Imaging and Other Noninvasive Techniques in Determining Adipose Tissue Mass -- Generation of Adipose Tissue-Specific Transgenic Mouse Models -- Angiogenesis in Adipose Tissue -- Adipose Organ Nerves Revealed by Immunohistochemistry# -- Human Adipose Tissue Blood Flow and Micromanipulation of Human Subcutaneous Blood Flow -- Studies of Thermogenesis and Mitochondrial Function in Adipose Tissues -- Application of Lipidomics and Metabolomics to the Study of Adipose Tissue -- Applications of Proteomics to the Study of Adipose Tissue -- Application of DNA Microarray to the Study of Human Adipose Tissue/Cells -- Differentiation of Adipose Stem Cells -- Methods That Resolve Different Contributions of Clonal Expansion to Adipogenesis in 3T3-L1 and C3H10T1/2 Cells -- Explant Cultures of White Adipose Tissue -- Isolation and Culture of Preadipocytes from Rodent White Adipose Tissue -- Flow Cytometry on the Stromal-Vascular Fraction of White Adipose Tissue -- Application of Electrophoretic Mobility Shift Assay and Chromatin Immunoprecipitation in the Study of Transcription in Adipose Cells -- Application of RNA Interference Techniques to Adipose Cell Cultures -- RNA Isolation and Real-Time Quantitative RT-PCR -- Study of Adipose Tissue Gene Expression by In Situ Hybridization -- Application of Immunocytochemistry and Immunofluorescence Techniques to Adipose Tissue and Cell Cultures -- Determination of Lipolysis in Isolated Primary Adipocytes -- Study of Glucose Uptake in Adipose Cells -- Measurement of Phosphoinositide 3-Kinase and Its Products to Study Adipogenic Signal Transduction.
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|a Since the publication of the first edition, the techniques and experimental approaches employed in the study of adipose tissue have undergone radical expansion and revision. In Adipose Tissue Protocols: Second Edition, several updated chapters are combined with many new contributions from internationally recognized experts covering both conventional and state-of-the-art methodologies as well as whole animal, cellular and molecular approaches which have emerged in or been extended to adipose tissue research over the past few years. As a volume in the highly sucessful Methods in Molecular Biology™ series, each chapter includes an introduction to its subject matter, a list of necessary materials, step-by-step, readily reproducible laboratory protocols, and a Notes section, which provides invaluable tips and tricks to safeguard against potential mishaps and pitfalls. Cutting-edge and comprehensive, Adipose Tissue Protocols: Second Edition is an essential manual for newcomers to this burgeoning area as well as a valuable addition to the laboratories and offices of more experienced researchers.
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|a Cytology.
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|a Human physiology.
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|a Endocrinology.
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|a Medicine
|x Research.
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|a Biology
|x Research.
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|a Cell Biology.
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|a Human Physiology.
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|a Endocrinology.
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|a Biomedical Research.
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|a Yang, Kaiping.
|e editor.
|4 edt
|4 http://id.loc.gov/vocabulary/relators/edt
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|a SpringerLink (Online service)
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|t Springer Nature eBook
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|i Printed edition:
|z 9781588299161
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|i Printed edition:
|z 9781617378447
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| 830 |
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|a Methods in Molecular Biology,
|x 1940-6029 ;
|v 456
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|u https://doi.org/10.1007/978-1-59745-245-8
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|a ZDB-2-PRO
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| 950 |
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|a Springer Protocols (Springer-12345)
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