Phosphodiesterase Methods and Protocols
| Korporacja: | |
|---|---|
| Kolejni autorzy: | |
| Streszczenie: | 336 p. 93 illus., 1 illus. in color. text |
| Język: | angielski |
| Wydane: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2005.
|
| Wydanie: | 1st ed. 2005. |
| Seria: | Methods in Molecular Biology,
307 |
| Hasła przedmiotowe: | |
| Dostęp online: | https://doi.org/10.1385/1592598390 |
| Format: | Elektroniczne Książka |
Spis treści:
- Study of Cyclic Adenosine Monophosphate Microdomains in Cells
- High-Resolution Measurements of Cyclic Adenosine Monophosphate Signals in 3D Microdomains
- Cygnets
- High-Throughput Screening of Phosphodiesterase Activity in Living Cells
- Assessment of Phosphodiesterase Isozyme Contribution in Cell and Tissue Extracts
- Localization of the Cyclic Guanosine 3?,5?-Monophosphate- Hydrolyzing Phosphodiesterase Type 9 in Rat Brain by Nonradioactive In Situ Hybridization
- Determination of Ca2+/Calmodulin-Stimulated Phosphodiesterase Activity in Intact Cells
- Adenovirus-Mediated Overexpression of Murine Cyclic Nucleotide Phosphodiesterase 3B
- Identification of Promoter Elements in the 5?-Flanking Region of Murine Cyclic Nucleotide Phosphodiesterase 3B Gene
- Purification of PDE6 Isozymes From Mammalian Retina
- Cyclic Guanosine 5?-Monophosphate Binding to Regulatory GAF Domains of Photoreceptor Phosphodiesterase
- Renaturation of the Catalytic Domain of PDE4A Expressed in Escherichia coli as Inclusion Bodies
- Determining the Subunit Structure of Phosphodiesterases Using Gel Filtration and Sucrose Density Gradient Centrifugation
- Crystallization of Cyclic Nucleotide Phosphodiesterases
- Generation of PDE4 Knockout Mice by Gene Targeting
- Immunoprecipitation of PDE2 Phosphorylated and Inactivated by an Associated Protein Kinase
- Investigation of Extracellular Signal-Regulated Kinase 2 Mitogen-Activated Protein Kinase Phosphorylation and Regulation of Activity of PDE4 Cyclic Adenosine Monophosphate-Specific Phosphodiesterases
- Radiolabeled Ligand Binding to the Catalytic or Allosteric Sites of PDE5 and PDE11
- Analysis of Dimerization Determinants of PDE6 Catalytic Subunits
- Interactions Between Catalytic and Inhibitory Subunits of PDE6
- Purification,Reconstitution on Lipid Vesicles, and Assays of PDE6 and Its Activator G Protein, Transducin.