Adenovirus Methods and Protocols

書目詳細資料
企業作者: SpringerLink (Online service)
其他作者: Wold, William S. M. (Editor)
總結:XIII, 352 p. 72 illus.
text
語言:英语
出版: Totowa, NJ : Humana Press : Imprint: Humana, 1999.
版:1st ed. 1999.
叢編:Methods in Molecular Medicine, 21
主題:
在線閱讀:https://doi.org/10.1385/0896035514
格式: 電子 圖書
書本目錄:
  • Preparation and Titration of CsCl-Banded Adenovirus Stock
  • Construction of Mutations in the Adenovirus Early Region 3 (E3) Transcription Units
  • Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants
  • Manipulation of Early Region 4
  • Adenovirus DNA Packaging
  • Methods for Creating and Analyzing Adenovirus Vectors that Express Proteins that Act on the Viral Genome
  • Construction of Mouse Adenovirus Type 1 Mutants
  • Generation of Adenovirus-Specific Cytotoxic T Lymphocytes
  • Measurement of Macrophage-Mediated Cytolysis of Adenovirus-Infected Cells
  • Measurement of Tumor Necrosis Factor (TNF) Lysis of Adenovirus-Infected Cells
  • Role of Natural Killer (NK) Cells in Immunity to Adenoviruses
  • Determination of the Transforming Activities of Adenovirus Oncogenes
  • Cell Survival and Apoptosis Assays for E1B 19K and Bcl-2 Family Members
  • In Vitro Transcription
  • Cell Microinjection
  • Immunoprecipitation of E1 A-Containing Protein Complexes
  • Preparation of Splicing-Competent Nuclear Extracts from Adenovirus-lnfected Cells
  • Adenovirus Entry into Cells
  • Simultaneous Detection of RNA and Proteins in Adenovirus-Infected Cells by Fluorescence In Situ Hybridization (FISH) and lmmunostaining
  • Characterization of the Adenovirus Fiber Protein
  • Large-Scale Purification and Crystallization of Adenovirus Hexon
  • Adenovirus Protease
  • Methods for Growth and Purification of Enteric Adenovirus Type 40
  • Transfection Complexes Generated with Adenovirus and Polyethylenimine-Condensed DNA
  • Phylogenetic Analysis of Adenovirus Sequences.