Adenovirus Methods and Protocols
| Institution som forfatter: | |
|---|---|
| Andre forfattere: | |
| Summary: | XIII, 352 p. 72 illus. text |
| Sprog: | engelsk |
| Udgivet: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1999.
|
| Udgivelse: | 1st ed. 1999. |
| Serier: | Methods in Molecular Medicine,
21 |
| Fag: | |
| Online adgang: | https://doi.org/10.1385/0896035514 |
| Format: | Electronisk Bog |
Indholdsfortegnelse:
- Preparation and Titration of CsCl-Banded Adenovirus Stock
- Construction of Mutations in the Adenovirus Early Region 3 (E3) Transcription Units
- Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants
- Manipulation of Early Region 4
- Adenovirus DNA Packaging
- Methods for Creating and Analyzing Adenovirus Vectors that Express Proteins that Act on the Viral Genome
- Construction of Mouse Adenovirus Type 1 Mutants
- Generation of Adenovirus-Specific Cytotoxic T Lymphocytes
- Measurement of Macrophage-Mediated Cytolysis of Adenovirus-Infected Cells
- Measurement of Tumor Necrosis Factor (TNF) Lysis of Adenovirus-Infected Cells
- Role of Natural Killer (NK) Cells in Immunity to Adenoviruses
- Determination of the Transforming Activities of Adenovirus Oncogenes
- Cell Survival and Apoptosis Assays for E1B 19K and Bcl-2 Family Members
- In Vitro Transcription
- Cell Microinjection
- Immunoprecipitation of E1 A-Containing Protein Complexes
- Preparation of Splicing-Competent Nuclear Extracts from Adenovirus-lnfected Cells
- Adenovirus Entry into Cells
- Simultaneous Detection of RNA and Proteins in Adenovirus-Infected Cells by Fluorescence In Situ Hybridization (FISH) and lmmunostaining
- Characterization of the Adenovirus Fiber Protein
- Large-Scale Purification and Crystallization of Adenovirus Hexon
- Adenovirus Protease
- Methods for Growth and Purification of Enteric Adenovirus Type 40
- Transfection Complexes Generated with Adenovirus and Polyethylenimine-Condensed DNA
- Phylogenetic Analysis of Adenovirus Sequences.