Clinical Applications of PCR

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Lo, Y. M. Dennis (Editor)
Summary:X, 353 p.
text
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 1998.
Edition:1st ed. 1998.
Series:Methods in Molecular Medicine, 16
Subjects:
Online Access:https://doi.org/10.1385/0896034992
Format: Electronic Book
Table of Contents:
  • to the Polymerase Chain Reaction
  • Setting Up a PCR Laboratory
  • General Methodology
  • Amplification from Archival Materials
  • Quantitative PCR
  • Mutation Screening Using PCR-SSCP
  • Multiple Fluorescence-Based PCR-SSCP Analysis with Primer-, Post-, and Internal-Labeling
  • The Amplification Refractory Mutation System
  • Artificial Restriction Fragment Length Polymorphism (A-RFLP) Analysis
  • Long Range PCR
  • Generation of Labeled Probes by PCR
  • PCR for the Detection of Minority DNA Populations
  • In Situ Amplification
  • Sequencing of PCR Products
  • Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)
  • Clinical Application
  • PCR Amplification of Minisatellite DNA for the Detection of Mixed Chimerism After Bone Marrow Transplantation
  • Diagnosis of Mitochondrial Disorders Using the PCR
  • PCR Analysis of CD44 Variants in Tumors
  • Detection of Circulating Solid Tumor Cells by Reverse Transcriptase Polymerase Chain Reaction
  • Detection of Circulating Tumor Cells Using lmmunobead-PCR
  • Single Cell PCR
  • PCR-Based Noninvasive Prenatal Diagnosis Using Fetal Cells in Maternal Circulation
  • Noninvasive Prenatal Diagnosis Using a Single Fetal Nucleated Erythrocyte Isolated by Micromanipulation from Maternal Blood
  • Diagnosis of Chromosomal Aneuploidies Using Quantitative Fluorescent PCR
  • Detection of the Hepatitis C Virus by RT-PCR
  • The Application of PCR to the Detection of M. tuberculosis in Sputum Samples
  • Microsatellite Analysis in Human Disease.