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|a 9781592595631
|9 978-1-59259-563-1
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|a 10.1385/089603397X
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|a Protein Synthesis
|h [electronic resource] :
|b Methods and Protocols /
|c edited by Robin Martin.
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|a 1st ed. 1998.
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1998.
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|a XIV, 442 p.
|b online resource.
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|a text
|b txt
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|a online resource
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|b PDF
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|a Methods in Molecular Biology,
|x 1940-6029 ;
|v 77
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|a Analyses of Ribosome Distribution During In Vitro Translation -- Allele-Specific Priming in the Mapping of rRNA -- Permeabilized Mammalian Cells as a System for Protein Synthesis -- The Measurement of Processivity Errors in Protein Synthesis -- A Biosynthetic Approach for the Incorporation of Unnatural Amino Acids into Proteins -- The Analysis of Translational Activity Using a Reporter Gene Constructed from Repeats of an Antibody-Binding Domain from Protein A -- In Vitro Engineering Using Acyl-Derivatized tRNAs -- In Vitro Engineering Using Synthetic tRNAs with Altered Anticodons Including Four-Nucleotide Anticodons -- Analysis of rRNA Function Using Specialized Ribosomes -- Determination of the Peptide Elongation Rate In Vivo -- The Measurement of Missense Errors During In Vivo Protein Synthesis -- Heelprinting Analysis of In Vivo Ribosome Pause Sites -- Analysis of elF-2? Kinases in Yeast -- Continuous-Flow Cell-Free Translation, Transcription-Translation, and Replication-Translation Systems -- Analysis of Translational Activity of Extracts Derived from Oocytes and Eggs of Xenopus laevis -- A Fractionated Reticulocyte Lysate System for Studies on Protein Synthesis Initiation Factors -- Measurement of Ribosomal Accuracy and Proofreading in E. coli Burst Systems -- Measurement of Rate of Protein Synthesis In Vitro -- In Vivo Mutational Analysis of Ribosomal RNA in Saccharomyces cerevisiae -- Genetic Selection of rRNA Mutations -- Toeprinting Assays -- Mapping of Pseudouridine Residues in RNA to Nucleotide Resolution -- Chemical and Enzymatic Probing of Antibiotic-Ribosome Complexes -- Protein Engineering with Nonstandard Amino Acids -- Internal Ribosome Entry Sites Tests with Circular mRNAs -- Gel Retardation and UV-Crosslinking Assays to Detect Specific RNA-Protein Interactions in the 5? or3? UTRs of Translationally Regulated mRNAs -- Translational Control by Repressor Proteins Binding to the 5?UTR of mRNAs -- Identification and Analysis of Frameshift Sites -- Synthesis and Site-Specific Binding of Thioated tRNAs to Probe Ribosome-tRNA Interactions.
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|a The synthesis of proteins from 20 or so constituent amino acids according to a strictly defined code with an accuracy of better than 1 in 10,000 at most loca tions is arguably the most complex task performed by cells. Protein Synthesis collects together methods and protocols covering a range of different approaches towards understanding how the cellular machinery accomplishes this task and how these ftinctions might be harnessed by the biotechnology industry to generate novel and useful proteins. The era in which the components of the translational machinery were being catalogued is over. This volume gathers together protocols that focus on preserving and describing the dynamic function as closely as possible. The need to understand exactly how ribosomes are positioned on messages or where tRNA molecules, translation factors, or control proteins are bound, has been appreciated by many of the authors. Several chapters that explore the fidelity and processivity of translation reflect this belief. Moreover, the fundamental importance of rRNA at the heart of the ribosome is a strong theme in a number of the protocols. These articles include in vitro and in vivo systems from bacterial, fungal, plant, and animal systems. Overall, Protein Synthesis might be characterized by the novelty of the approaches employed to illuminate the inner workings of the protein synthetic machinery as well as by the inventiveness of the attempts to harness these reactions for biotechnological applications.
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|a Accessibility summary: This PDF is not accessible. It is based on scanned pages and does not support features such as screen reader compatibility or described non-text content (images, graphs etc). However, it likely supports searchable and selectable text based on OCR (Optical Character Recognition). Users with accessibility needs may not be able to use this content effectively. Please contact us at accessibilitysupport@springernature.com if you require assistance or an alternative format.
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|a Inaccessible, or known limited accessibility
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|a No reading system accessibility options actively disabled
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|a Publisher contact for further accessibility information: accessibilitysupport@springernature.com
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|a Biochemistry.
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|a Biochemistry.
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|a Martin, Robin.
|e editor.
|4 edt
|4 http://id.loc.gov/vocabulary/relators/edt
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|a SpringerLink (Online service)
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|t Springer Nature eBook
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|i Printed edition:
|z 9780896033979
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|i Printed edition:
|z 9781617370250
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|i Printed edition:
|z 9781489942746
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|a Methods in Molecular Biology,
|x 1940-6029 ;
|v 77
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|u https://doi.org/10.1385/089603397X
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|a Springer Protocols (Springer-12345)
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