PCR Sequencing Protocols
| 企业作者: | |
|---|---|
| 其他作者: | |
| 总结: | XI, 221 p. 24 illus. text |
| 语言: | 英语 |
| 出版: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1996.
|
| 版: | 1st ed. 1996. |
| 丛编: | Methods in Molecular Biology,
65 |
| 主题: | |
| 在线阅读: | https://doi.org/10.1385/0896033449 |
| 格式: | 电子 图书 |
书本目录:
- Preparation and Analysis of DNA Sequencing Gels
- Purification of PCR Products from Agarose Gels for Direct Sequencing
- Enzymatic Fluorescence and Biotin Labeling of Primers for PCR Sequencing
- Direct Sequencing of Double-Stranded PCR Products with the Sequenase Kit and [?-35 S] dATP
- Direct Sequencing by Thermal Asymmetric PCR
- Rapid Sequencing of cDNA Clones Direct Sequencing Using Sequential Linear/Asymmetric PCR
- Direct Sequencing of PCR Products Using Chemiluminescent Detection
- Direct DNA Sequencing of PCR Products Using Magnetic Beads
- Affinity Capture and Solid-Phase Sequencing of Biotinylated PCR Products
- Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing
- Nonradioactive PCR Sequencing Using Digoxigenin
- Silver Sequencing™
- Direct Sequencing of PCR Products with DNA-Binding Proteins
- PCR Sequencing with the Aid of Detergents
- Direct Sequencing with Highly Degenerate and lnosine-Containing Primers
- Determination of Unknown Genomic Sequences Without Cloning
- DNA Sequencing by the Chemical Method
- Direct PCR Sequencing with Denaturants (Formamide)
- Efficient PCR Production of Single-Stranded DNA Sequencing Templates
- Preparation and Direct Automated Cycle Sequencing of PCR Products
- Solid-Phase Automated Sequencing of PCR-Amplified Genomic DNA
- Cloning PCR Products for Sequencing in M13 Vectors
- Sequencing PCR Products Cloned into M13 Vectors
- Genomic Amplification with Transcript Sequencing (GAWTS)
- DNA Rescue by the Vectorette Method
- Sequencing of (dA:dT) Cloned Mixed PCR Products from Microbial Populations.