Directed Enzyme Evolution Screening and Selection Methods /
| Autor corporatiu: | |
|---|---|
| Altres autors: | , |
| Sumari: | XV, 383 p. text |
| Idioma: | anglès |
| Publicat: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2003.
|
| Edició: | 1st ed. 2003. |
| Col·lecció: | Methods in Molecular Biology,
230 |
| Matèries: | |
| Accés en línia: | https://doi.org/10.1385/1592593968 |
| Format: | Electrònic Llibre |
Taula de continguts:
- Genetic Selections
- Genetic Complementation Protocols
- Use of Pol I-Deficient E. coli for Functional Complementation of DNA Polymerase
- Selection of Novel Eukaryotic DNA Polymerases by Mutagenesis and Genetic Complementation of Yeast
- Autogene Selections
- Selection for Soluble Proteins via Fusion with Chloramphenicol Acetyltransferase
- Proside
- Minimization of Proteins by Random Fragmentation and Selection
- Screens for Enzymes
- Evaluating a Screen and Analysis of Mutant Libraries
- Screening Mutant Libraries in Saccharomyces cerevisiae
- Solid-Phase Screening Using Digital Image Analysis
- Screening for Thermostability
- High-Throughput Screening of Mutant ?-Amylase Libraries for Increased Activity at 129°C
- High-Throughput Carbon Monoxide Binding Assay for Cytochromes P450
- High-Throughput Screen for Aromatic Hydroxylation
- Colorimetric Screen for Aliphatic Hydroxylation by Cytochrome P450 Using p-Nitrophenyl-Substituted Alkanes
- High-Throughput Screens Based on NAD(P)H Depletion
- High-Throughput Tetramethylbenzidine (TMB) Screen for Peroxidases
- Screen for Oxidases by Detection of Hydrogen Peroxide with Horseradish Peroxidase
- Colorimetric Dehydrogenase Screen Based on NAD(P)H Generation
- Colorimetric Assays for Screening Laccases
- pH Sensing Agar Plate Assays for Esterolytic Enzyme Activity
- A pH-Indicator-Based Screen for Hydrolytic Haloalkane Dehalogenase
- Detection of Aromatic ?-Hydroxyketones with Tetrazolium Salts
- Selection of Heat-Stable Clostridium cellulovorans Cellulases After In Vitro Recombination
- Screening and Selection Strategies for Disulfide Isomerase Activity
- An Overview of High-Throughput Screening Systems for Enantioselective Enzymatic Transformations
- Select Protocols of High-Throughput ee-Screening Systems forAssaying Enantioselective Enzymes
- Directed Evolution of the Substrate Specificities of a Site-Specific Recombinase and an Aminoacyl-tRNA Synthetase Using Fluorescence-Activated Cell Sorting (FACS)
- Calmodulin-Tagged Phage and Two-Filter Sandwich Assays for the Identification of Enzymatic Activities
- High-Throughput FACS Method for Directed Evolution of Substrate Specificity
- Improving Protein Folding Efficiency by Directed Evolution Using the GFP Folding Reporter.