Protein Kinase Protocols

Մատենագիտական մանրամասներ
Համատեղ հեղինակ: SpringerLink (Online service)
Այլ հեղինակներ: Reith, Alastair D. (Խմբագիր)
Ամփոփում:XIII, 365 p.
text
Լեզու:անգլերեն
Հրապարակվել է: Totowa, NJ : Humana Press : Imprint: Humana, 2001.
Հրատարակություն:1st ed. 2001.
Շարք:Methods in Molecular Biology, 124
Խորագրեր:
Առցանց հասանելիություն:https://doi.org/10.1385/1592590594
Ձևաչափ: Էլեկտրոնային Գիրք
Բովանդակություն:
  • Protein Kinase-Mediated Signaling Networks
  • Cloning Protein Tyrosine Kinases by Screening cDNA Libraries with Antiphosphotyrosine Antibodies
  • Immunoprecipitation and Western Blotting of Phosphotyrosine-Containing Proteins
  • Two-Dimensional Phosphoamino Acid Analysis
  • Two-Dimensional Phosphopeptide Mapping of Receptor Tyrosine Kinases
  • Identification of the Sites of Phosphorylation in Proteins Using High Performance Liquid Chromatography and Mass Spectrometry
  • Phosphorylation of Smad Signaling Proteins by Receptor Serine/Threonine Kinases
  • Assays for Mitogen-Activated Protein Kinase (MARK) Subtypes and MARK Activating Protein Kinase-2 (MAPKAP K-2) Using a Common Cell Lysate
  • JAK-Mediated Phosphorylation and Activation of STAT Signaling Proteins
  • Assays for Glycogen Synthase Kinase-3(GSK-3)
  • Cyclin-Dependent Kinases and Cyclin-Dependent Kinase Inhibitors
  • Protein Histidine Kinase
  • Cloning and Expression of Recombinant HEXA-HIS Tagged ZAP-70 Using the Baculovirus System
  • Analysis of Protein Kinase Subcellular Localization by Visualization of GFP Fusion Proteins
  • Detection of Phosphorylation-Dependent Interactions by Far-Western Gel Overlay
  • Purification of Tyrosine-Phosphorylated Proteins by Immunoaffinity Chromatography and Direct Cloning of Their cDNAs from Bacterial Expression Libraries
  • Identification of Receptor Tyrosine Kinase (RTK) Substrates by the Cloning of ReceptorTargets (CORT) Strategy
  • Identification of Receptor Tyrosine Kinase Associating Proteins Using the Yeast Two-Hybrid System
  • cDNA Expression Cloning and Characterization of Phosphorylation Dependent Protein Interactors Using the Yeast Tribrid System
  • Analysis of SH2 Domain - Phosphopeptide Interactions by Isothermal Titration Calorimetry and Surface Plasmon Resonance
  • Cloning and Characterization of RTK Ligands Using Receptor-Alkaline Phosphatase Fusion Proteins
  • Isolation and Characterization of “Orphan-RTK” Ligands Using an Integrated Biosensor Approach.