Macrophage Migration Inhibitory Factor Methods and Protocols /
| Korporativní autor: | |
|---|---|
| Další autoři: | , |
| Shrnutí: | XI, 265 p. 43 illus., 34 illus. in color. text |
| Jazyk: | angličtina |
| Vydáno: |
New York, NY :
Springer US : Imprint: Humana,
2020.
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| Vydání: | 1st ed. 2020. |
| Edice: | Methods in Molecular Biology,
2080 |
| Témata: | |
| On-line přístup: | https://doi.org/10.1007/978-1-4939-9936-1 |
| Médium: | Elektronický zdroj Kniha |
Obsah:
- Studying the Pro-Migratory Effects of MIF
- In Vitro Approaches for Investigating the Influence of MIF on Leukocyte-Endothelial Cell Interactions under Flow Conditions
- Using Intravital Microscopy to Study the Role of MIF in Leukocyte Trafficking In Vivo
- Analysis of Secreted MIF from Cultured Murine Bone Marrow-Derived Immune Cells
- Measuring MIF in Biological Fluids
- Flow Cytometry Phenotyping of Bone Marrow-Derived Macrophages from Wildtype and MIF KO Mice
- Genotyping Two Promoter Polymorphisms in the MIF Gene: a –794 CATT5-8 Microsatellite Repeat and a –173 G/C SNP
- Staining MIF in Cells for Confocal Microscopy
- Microscopy Methods for Imaging MIF and its Interaction Partners
- Co-Immunoprecipitation (Co-IP) of Macrophage Migration Inhibitory Factor (MIF)
- Concurrent Immunohistochemical Localization and Western Blot Analysis of the MIF Receptor, CD74, in Formalin-Fixed, Paraffin-Embedded Tissue
- Methods to Determine the Effects of MIF on In Vitro Osteoclastogenesis using Murine Bone Marrow-Derived Cells and Human Peripheral Blood Mononuclear Cells (PBMCs)
- Inducing and Inhibiting Autophagy to Investigate its Interactions with MIF
- Assays for Measuring the Role of MIF in NLRP3 Inflammasome Activation
- Assays for Inducing and Measuring Cell Death to Detect Macrophage Migration Inhibitory Factor (MIF) Release
- Assessing the Role of MIF in Plasmodium spp. Infections using Ex Vivo Models
- The Effect of Macrophage Migration Inhibitory Factor on Intestinal Permeability: FITC-Dextran Serum Measurement and Transmission Electron Microscopy
- Investigating MIF in Mouse Models of Severe Corticosteroid-Resistant Neutrophilic Asthma
- Investigating MIF in Mouse Model of Gout
- 5´and 3’ RACE Method to Obtain Full-Length 5' and 3' Ends of Ciona robusta Macrophage Migration Inhibitory Factors MIF 1 and MIF 2 cDNA
- Purification of Antibodies against Entamoeba histolytica MIF and their use in Analyzing Human and Mouse Samples
- Studying Plant MIF/D-DT-Like Genes and Proteins (MDLs).