Chimeric RNA Methods and Protocols /

Bibliografski detalji
Autor kompanije: SpringerLink (Online service)
Daljnji autori: Li, Hui (Urednik), Elfman, Justin (Urednik)
Sažetak:XII, 262 p. 56 illus., 52 illus. in color.
text
Jezik:engleski
Izdano: New York, NY : Springer US : Imprint: Humana, 2020.
Izdanje:1st ed. 2020.
Serija:Methods in Molecular Biology, 2079
Teme:
Online pristup:https://doi.org/10.1007/978-1-4939-9904-0
Format: Elektronički e-knjiga
Sadržaj:
  • Prediction, Characterization, and In Silico Validation of Chimeric RNAs
  • Identification of Fusion Transcripts from Unaligned RNA-Seq Reads Using ChimeRScope
  • Identification of Chimeric RNAs Using RNA-Seq Reads and Protein-Protein Interactions of Translated Chimeras
  • Gene Fusion Discovery with INTEGRATE
  • Case Study: Systematic Detection and Prioritization of Gene Fusions in Cancer by RNA-Seq: A DIY Toolkit
  • Detection and Measurement of Chimeric RNAs by RT-PCR
  • Detection of Group II Intron-Generated Chimeric mRNAs in Bacterial Cells
  • RNase Protection Assay
  • Validation of Chimeric Fusion Peptides Using Proteomics Data
  • NanoString nCounter Technology: High-Throughput RNA Validation
  • Knockdown of Chimeric RNA by RNAi
  • Overexpression of Chimeric RNA by Retroviral Transduction
  • Separation of Nuclear and Cytoplasmic Fractions for Chimeric RNA Characterization
  • Confirmation of Transcriptional Read-Through Events byRT-PCR
  • Validating Gene Fusion as the Source of Chimeric RNAs
  • Chimeric RNA and Exosomes-Based Liquid Biopsy
  • SMaRT for Therapeutic Purposes
  • Case Study: Landscape of Chimeric RNAs in Bladder Cancer
  • Case Study: The Recurrent Fusion RNA DUS4L-BCAP29 in Non-Cancer Human Tissues and Cells.